Purchase of an ion trap mass spectrometer

描述（由申请人提供）：申请资金用于购买ThermoFinnigan LTQ Orbitrap质谱仪，该质谱仪将安装在Wistar研究所蛋白质组学核心设施中。该仪器将提供急需的增强能力，以在复杂样品中以高置信度鉴定蛋白质和翻译后修饰。由8名研究者组成的主要用户组将使用75%的仪器时间，8名次要用户将占仪器使用的另外21%。主要和次要用户组中的大多数研究人员都有多个NIH资助的研究项目，这些项目将使用该仪器。因此，一个线性离子阱质谱仪，是完全提供给主要用户组是迫切需要的，并将大大推进NIH资助的研究在Wistar研究所。购买LTQ Orbitrap质谱仪是强烈的首选，而不是一个独立的LTQ仪器，因为LTQ Orbitrap也将提供高分辨率和高精度的测量前体和碎片离子质量。这将极大地提高复杂混合物中低丰度组分的鉴定，提高置信度，并且还应提高翻译后修饰鉴定的置信度。即使增加了所要求的长期探测器轨道阱，对2007年预期工作量的保守预测表明，这一仪器和现有的长期探测器都需要接近满负荷运行，每天24小时，每周7天，才能满足需求。所要求的仪器将被纳入蛋白质组学核心设施，该设施在过去6年中为许多学术研究人员提供了LC-MS/MS分析服务。2005年，共有30个不同的实验室使用了这些服务，预计今后的用户数量也将类似。Wistar研究所承诺在所要求的质谱仪的使用寿命内为其提供住房、维护和人员配备，并由研究所所长兼首席执行官的随附信函予以核实。

DESCRIPTION (provided by applicant): Funds are requested to purchase a ThermoFinnigan LTQ Orbitrap mass spectrometer that will be housed in the Wistar Institute Proteomics Core Facility. This instrument will provide critically needed increased capacity to identify proteins and posttranslational modifications with high confidence in complex samples. A major user group of 8 investigators will utilize 75% of instrument time and 8 minor users will account for an additional 21% of instrument use. Most investigators in both the major and minor user groups have multiple NIH funded research projects that will utilize this instrument. Hence, a linear ion trap mass spectrometer that is fully available to the major user group is critically needed and will greatly advance NIH funded research at the Wistar Institute. The purchase of an LTQ Orbitrap mass spectrometer is strongly preferred rather than a stand- alone LTQ instrument because the LTQ Orbitrap will also provide high resolution and high accuracy measurements of precursor and fragment ion masses. This will greatly enhance the identification of low abundance components in complex mixtures with improved confidence, and it should also improve the confidence of identifications of posttranslational modifications. Even with the addition of the requested LTQ Orbitrap, conservative projections of anticipated workload for 2007 indicate that both this instrument and an existing LTQ will need to be operated near full capacity, 24 hours per day, 7 days a week to keep up with demand. The requested instrument will be incorporated into a Proteomics Core Facility that has provided LC- MS/MS analysis services for the past 6 years to many academic investigators. In 2005, a total of 30 different laboratories used these services and similar numbers of users are anticipated for the future. The Wistar Institute is committed to housing, maintaining and staffing the requested mass spectrometer for its useful lifetime as verified by an accompanying letter from the Institute President and CEO.