Purchase of an ion trap mass spectrometer

购买离子阱质谱仪

• 批准号: 7217102
• 负责人: DAVID W. SPEICHER
• 金额: $ 50万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217102
• 关键词: Accounting; Commit; Complex; Complex Mixtures; Core Facility; Funding; Funding Applicant; Future; Hour; Housing; Institutes; Ions; Laboratories; Letters; Measurement; Minor; Numbers; Post-Translational Protein Processing; Proteins; Proteomics; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Services; Time; United States National Institutes of Health; Week; Workload; day; improved; instrument; mass spectrometer

DESCRIPTION (provided by applicant): Funds are requested to purchase a ThermoFinnigan LTQ Orbitrap mass spectrometer that will be housed in the Wistar Institute Proteomics Core Facility. This instrument will provide critically needed increased capacity to identify proteins and posttranslational modifications with high confidence in complex samples. A major user group of 8 investigators will utilize 75% of instrument time and 8 minor users will account for an additional 21% of instrument use. Most investigators in both the major and minor user groups have multiple NIH funded research projects that will utilize this instrument. Hence, a linear ion trap mass spectrometer that is fully available to the major user group is critically needed and will greatly advance NIH funded research at the Wistar Institute. The purchase of an LTQ Orbitrap mass spectrometer is strongly preferred rather than a stand- alone LTQ instrument because the LTQ Orbitrap will also provide high resolution and high accuracy measurements of precursor and fragment ion masses. This will greatly enhance the identification of low abundance components in complex mixtures with improved confidence, and it should also improve the confidence of identifications of posttranslational modifications. Even with the addition of the requested LTQ Orbitrap, conservative projections of anticipated workload for 2007 indicate that both this instrument and an existing LTQ will need to be operated near full capacity, 24 hours per day, 7 days a week to keep up with demand. The requested instrument will be incorporated into a Proteomics Core Facility that has provided LC- MS/MS analysis services for the past 6 years to many academic investigators. In 2005, a total of 30 different laboratories used these services and similar numbers of users are anticipated for the future. The Wistar Institute is committed to housing, maintaining and staffing the requested mass spectrometer for its useful lifetime as verified by an accompanying letter from the Institute President and CEO.

描述(由申请人提供)：请提供资金购买一台ThermoFinnigan LTQ Orbitrap质谱仪，该质谱仪将安装在Wistar研究所蛋白质组学核心设施中。这一仪器将提供急需的更多能力，以在复杂样本中高度可信地识别蛋白质和翻译后修饰。由8名调查人员组成的主要用户组将利用75%的仪器时间，8名次要用户将占额外21%的仪器使用时间。主要和次要用户群体中的大多数研究人员都有多个由NIH资助的研究项目，这些项目将利用这一工具。因此，迫切需要一种对主要用户群体完全可用的线性离子陷阱质谱仪，这将极大地推动美国国立卫生研究院资助的维斯塔尔研究所的研究。购买LTQ Orbitrap质谱仪比购买独立的LTQ仪器更可取，因为LTQ Orbitrap还将提供对前体和碎片离子质量的高分辨率和高精度测量。这将大大增强对复杂混合物中低丰度组分的识别，并提高置信度，也应提高翻译后修饰识别的置信度。即使增加了所要求的LTQ Orbitrap，对2007年预期工作量的保守预测表明，该仪器和现有的LTQ都需要在接近满负荷的情况下运行，一周7天，每天24小时，才能跟上需求。所要求的仪器将被并入蛋白质组学核心设施，该设施在过去6年为许多学术研究人员提供LC-MS/MS分析服务。2005年，共有30个不同的实验室使用这些服务，预计未来将有类似数量的用户。Wistar研究所致力于为所要求的质谱仪的使用寿命提供住房、维护和人员配备，研究所主席兼首席执行官的一封信证实了这一点。