CORE--TISSUE ACQUISITION AND CELL CULTURE

核心——组织采集和细胞培养

• 批准号: 6202603
• 负责人: WALTER E FINKBEINER
• 金额: $ 18.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6202603
• 关键词: biomedical facility; cystic fibrosis; human tissue; respiratory system; tissue /cell culture

Basic research toward understanding the pathobiology of cystic fibrosis requires a continuing and dependable source of cells, affected and unaffected by the disease. Thus, the primary objective of the Core Cell Culture Facility is to acquire airway tissues and culture airway surface epithelial and tracheobronchial gland cells. A tissue procurement system has been established for obtaining normal and cystic fibrosis airway tissue. Once obtained, surface epithelial tissue is enzymatically digested and liberated cells are established in primary culture. Tracheobronchial gland cells are isolated as acini and expanded through a single passage. Surface epithelial and gland cell cultures are evaluated for differentiated properties using light microscopy, electron microscopy and measurements of short circuit current and transpithelial resistance. Cells showings satisfactory features are distributed to investigators. If surplus cells are available, these are frozen for use later. Additionally, established cell lines routinely used by investigators are maintained in the Core Cell Culture Facility. The final aim of the Cell Culture Core Facility is to continue efforts to improve cultures, particularly those derived from tracheobronchial gland cells and to develop cell culture model systems applicable to the studies proposed by SCOR investigators. With respect to improving in vitro cell differentiation, both soluble factors (growth factors, hormones, chemicals) added to culture medium and insoluble factors (extracellular matrix components, air interface culture) are tested for their effects on airway gland cell growth and differentiation. Culture conditions that allow full expression of ion transport and mucus secretory function are two of the goals of this aim. However, the highest priority will be given to determining the culture conditions that promote serous gland cell differentiation.

了解囊性纤维化病理生物学的基础研究 需要持续和可靠的细胞来源，受影响和 没有受到这种疾病的影响。因此，核心单元的主要目标是 培养设施是获取呼吸道组织和培养呼吸道表面 上皮和气管支气管腺细胞。一种纸巾采购系统 已经建立了获得正常和囊性纤维化气道的方法 组织。一旦获得表面上皮组织，就会被酶消化 并在原代培养中建立了解放细胞。气管、支气管镜 腺细胞被分离成腺泡，并通过单个通道扩张。 对表面上皮细胞和腺体细胞培养进行评估 利用光学显微镜、电子显微镜和 短路电流和跨上皮阻力的测量。单元格 令人满意的特征被分发给调查人员。如果 多余的细胞是可用的，这些细胞被冷冻起来供以后使用。另外， 研究人员常规使用的已建立的细胞系在 核心细胞培养设施。细胞培养核心的最终目标 设施是继续努力改善文化，特别是那些 从气管、支气管腺细胞中提取细胞并进行细胞培养 适用于SCOR调查人员提出的研究的示范系统。 关于促进体外细胞分化，两者都可 添加到培养基中的因子(生长因子、激素、化学物质)和 不溶性因素(细胞外基质成分、空气界面培养) 测试它们对呼吸道腺体细胞生长的影响 差异化。允许充分表达离子的培养条件 转运和粘液分泌功能是这一目标的两个目标。 但是，最优先考虑的将是确定区域性 促进浆液腺细胞分化的条件。