CORE--CELL MODELS

核心--细胞模型

• 批准号: 7455091
• 负责人: WALTER E FINKBEINER
• 金额: $ 8.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7455091
• 关键词: Acinar Cell; Affect; Biological Models; California; Cell Count; Cell Culture Techniques; Cell Separation; Cell model; Cell physiology; Cells; Clinic; Collection; Condition; Core Facility; Cultured Cells; Cystic Fibrosis; Defect; Drug effect disorder; Epithelial; Epithelial Cells; Gland; Healthcare; In Vitro; Institution; Laboratories; Lead; Lung diseases; Maintenance; Mucous body substance; Nose; Patients; Pharmaceutical Preparations; Phenotype; Preparation; Primary Cell Cultures; Research; Research Personnel; Resources; Respiratory System; Running; Screening procedure; Serous; Services; Source; Structure; Surface; System; Testing; Tissue Procurements; Tissues; Tracheobronchial; airway epithelium; experience; programs; respiratory; tissue preparation; tool

Basic pharmacological research aimed at identifying drugs that may correct the cellular derangements that lead to the debilitating and often fatal lung disease associated with cystic fibrosis requires a continuing and dependable source of cystic fibrosis and "normal" respiratory tissues. Cell culture techniques that allow the propagation of respiratory epithelial cells and formation of differentiated cell culture model systems provide a necessary and powerful tool for testing candidate drugs. Thus, the primary objective of the Cell Models Core is the collection of CF and non-CF respiratory tract tissues and the preparation of differentiated cultures of the respiratory surface epithelial cells and the tracheobronchial submucosal gland cells. A secondary objective of the Cell Models Core is to identify cell culture conditions that permit full expression of differentiated cellular functions, particularly in cell cultures that have been expanded by serial propagation. A tissue procurement system has been established for obtaining both CF and control nasal and airway tissues. Procured tissue is enzymatically digested, and liberated surface epithelial and submucosal gland acinar cells are established as cell cultures. The surface epithelial cells are studied as primary cell cultures of highly differentiated cell sheets that mimic the structure and function of the native nasal and airway epithelium. To increase the number of cell sheets available for screening compounds, some cell cultures are also expanded by serial propagation. The initial primary cultures of the tracheobronchial gland cells are expanded and then seeded as secondary cultures for experimental use. The conditions in which the passaged gland cells are grown are manipulated to produce cells expressing either a mucous or serous cell phenotype. Having respiratory epithelial cells derived from both the mucosal surface and the submucosal gland cells allows investigators to test candidate drugs for action in the various respiratory tract cells most affected in cystic fibrosis.

基础药理学研究，旨在确定可能纠正细胞 导致与囊性肺炎相关的衰弱和通常致命的肺部疾病的错乱 纤维化需要持续可靠的囊性纤维化来源和“正常”的呼吸道 纸巾。细胞培养技术，允许呼吸道上皮细胞和 分化细胞培养模型体系的建立提供了必要而有力的工具 测试候选药物。因此，细胞模型核心的主要目标是收集CF型和非CF型呼吸道组织，并准备呼吸道表面上皮细胞和气管-支气管腺黏膜下腺细胞的分化培养。细胞模型核心的第二个目标是确定细胞培养条件，允许充分表达分化的细胞功能，特别是在通过连续繁殖扩大的细胞培养中。已经建立了一套组织采购系统，以获得CF和对照鼻腔和呼吸道组织。获取的组织被酶消化，释放的表面上皮细胞和粘膜下腺泡细胞被建立为细胞培养。表面上皮细胞被作为高度分化的细胞片的原代细胞培养物来研究，这种细胞片模仿天然鼻腔和呼吸道上皮的结构和功能。为了增加可用于筛选化合物的细胞片的数量，一些细胞培养物也通过连续繁殖进行扩展。对气管、支气管腺细胞的初始原代培养进行扩增，然后播种作为实验用的二次培养。培养传代的腺体细胞的条件被操纵，以产生表达粘液或浆液细胞表型的细胞。具有来源于粘膜表面和粘膜下腺细胞的呼吸道上皮细胞 允许研究人员测试候选药物在各种呼吸道细胞中的作用 患有囊性纤维症。