CORE--TISSUE ACQUISITION AND CELL CULTURE

核心——组织采集和细胞培养

• 批准号: 6110950
• 负责人: WALTER E FINKBEINER
• 金额: $ 18.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6110950
• 关键词: biomedical facility; cystic fibrosis; human tissue; respiratory system; tissue /cell culture

Basic research toward understanding the pathobiology of cystic fibrosis requires a continuing and dependable source of cells, affected and unaffected by the disease. Thus, the primary objective of the Core Cell Culture Facility is to acquire airway tissues and culture airway surface epithelial and tracheobronchial gland cells. A tissue procurement system has been established for obtaining normal and cystic fibrosis airway tissue. Once obtained, surface epithelial tissue is enzymatically digested and liberated cells are established in primary culture. Tracheobronchial gland cells are isolated as acini and expanded through a single passage. Surface epithelial and gland cell cultures are evaluated for differentiated properties using light microscopy, electron microscopy and measurements of short circuit current and transpithelial resistance. Cells showings satisfactory features are distributed to investigators. If surplus cells are available, these are frozen for use later. Additionally, established cell lines routinely used by investigators are maintained in the Core Cell Culture Facility. The final aim of the Cell Culture Core Facility is to continue efforts to improve cultures, particularly those derived from tracheobronchial gland cells and to develop cell culture model systems applicable to the studies proposed by SCOR investigators. With respect to improving in vitro cell differentiation, both soluble factors (growth factors, hormones, chemicals) added to culture medium and insoluble factors (extracellular matrix components, air interface culture) are tested for their effects on airway gland cell growth and differentiation. Culture conditions that allow full expression of ion transport and mucus secretory function are two of the goals of this aim. However, the highest priority will be given to determining the culture conditions that promote serous gland cell differentiation.

了解囊性纤维化病理生物学的基础研究 需要一个持续和可靠的细胞来源，受影响， 不受疾病的影响。因此，核心小组的主要目标是 培养设备用于获取气道组织并培养气道表面 上皮细胞和气管支气管腺细胞。一种组织获取系统， 已经建立了获得正常和囊性纤维化气道 组织.一旦获得，表面上皮组织被酶消化， 并在原代培养物中建立释放的细胞。气管 腺细胞作为腺泡分离并通过单次传代扩增。 评价表面上皮细胞和腺细胞培养物的 使用光学显微镜、电子显微镜和 短路电流和透皮电阻的测量。细胞 显示出令人满意的特征被分发给调查者。如果 剩余的细胞可用，这些细胞被冷冻以备后用。此外，本发明还 研究者常规使用的已建立的细胞系保存在 核心细胞培养设施细胞培养核心的最终目的 设施是继续努力改善文化，特别是那些 来源于气管支气管腺细胞并进行细胞培养 适用于SCOR研究者提出的研究的模型系统。 关于改善体外细胞分化，可溶性和非可溶性细胞都可以在体外分化。 添加到培养基中的因子（生长因子、激素、化学品）， 不溶性因子（细胞外基质成分，空气界面培养） 测试它们对气道腺细胞生长的影响， 分化允许离子完全表达的培养条件 运输和粘液分泌功能是该目标的两个目标。 但是，最高优先级将是确定文化 促进浆液腺细胞分化的条件。