SOLID STATE NMR STUDY OF PROTON TRANSPORT PATHWAY OF BACTERIORHODOPSIN

阳离子泵系统在许多细胞中扮演着重要的角色 细胞生长、运动和肿瘤形成等过程。尽管 它们的无处不在和重要性，矢量化的分子机制 人们对交通工具的理解还不够透彻。细菌视紫红质提供了一种强大的 阳离子迁移模型系统。它是一种膜结合蛋白 来自盐生盐生杆菌，起到了光驱动质子泵的作用。 从所做的工作到目前为止，我们知道它由七个一组组成 α螺旋和视网膜发色团与席夫碱结合 莱斯216岁。人们还知道，光循环至少包括五个 中间体。这项工作的目标是定义连通性 在参与转运途径的官能团和TO之间 确定这些连接性在光循环期间如何变化。这 将通过使用碳和氮的固态核磁共振来完成 检测到了二维氢交换。从一组 模型化合物，交换光谱的细节将是 已经成立了。然后，生色团/残基的变化 连接性将在光线适应的情况下进行研究 细菌视紫红质与质子运动的光中间体 光循环。

Cation pumping systems play important roles in many cellular processes such as cell growth, motility, and tumorigenesis. Despite their ubiquity and importance, the molecular mechanism for vectoral transport is not well understood. Bacteriorhodopsin provides a robust model system for cation transport. It is a membrane bound protein from Halobacterium salinarium that acts as a light-driven proton pump. From work done to date it is known that it comprises a bundle of seven alpha helices and a retinal chromophore bound by a Schiff base at Lys216. It is also know that the photocycle comprises at least five intermediates. The goal of this work is to define connectivities between functional groups involved in the transport pathway and to determine how these connectivities change during the photocycle. This will be accomplished by solid state NMR, using carbon and nitrogen detected two dimensional deuterium exchange. Starting with a set of model compounds, the details of the exchange spectra will be established. Then, the variations in the chromophore/residue connectivity will be investigated in the light adapted bacteriorhodopsin and the photo intermediates of the proton-motive photocycle.