SOLID STATE NMR STUDY OF PROTON TRANSPORT PATHWAY OF BACTERIORHODOPSIN
SOLID STATE NMR STUDY OF PROTON TRANSPORT PATHWAY OF BACTERIORHODOPSIN
批准号:
6355166
负责人:
BRETT A TOUNGE
金额:
$1.82万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-05-01 至 2001-04-30
中文摘要
阳离子泵送系统在许多细胞中起着重要的作用
英文摘要
Cation pumping systems play important roles in many cellular
processes such as cell growth, motility, and tumorigenesis. Despite
their ubiquity and importance, the molecular mechanism for vectoral
transport is not well understood. Bacteriorhodopsin provides a robust
model system for cation transport. It is a membrane bound protein
from Halobacterium salinarium that acts as a light-driven proton pump.
From work done to date it is known that it comprises a bundle of seven
alpha helices and a retinal chromophore bound by a Schiff base at
Lys216. It is also know that the photocycle comprises at least five
intermediates. The goal of this work is to define connectivities
between functional groups involved in the transport pathway and to
determine how these connectivities change during the photocycle. This
will be accomplished by solid state NMR, using carbon and nitrogen
detected two dimensional deuterium exchange. Starting with a set of
model compounds, the details of the exchange spectra will be
established. Then, the variations in the chromophore/residue
connectivity will be investigated in the light adapted
bacteriorhodopsin and the photo intermediates of the proton-motive
photocycle.
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SOLID STATE NMR STUDY OF PROTON TRANSPORT PATHWAY OF BACTERIORHODOPSIN
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批准号:6118708
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项目类别:
-
资助金额:$1.82万
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财政年份:1999
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负责人:BRETT A TOUNGE
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依托单位:
海外基金