SOLID STATE NMR STUDY OF PROTON TRANSPORT PATHWAY OF BACTERIORHODOPSIN

阳离子泵送系统在许多细胞中起着重要作用。 过程，如细胞生长、运动和肿瘤发生。 尽管 它们的普遍性和重要性， 运输不太清楚。 细菌视紫红质提供了一个强大的 模型系统的阳离子传输。 它是一种膜结合蛋白 盐生盐杆菌中的一种，它是一种光驱动的质子泵。 从迄今为止所做的工作来看，它包括一捆七个 α螺旋和由席夫碱结合的视网膜发色团， Lys216 还已知光循环包括至少五个 中间体的 这项工作的目标是定义连接性 参与运输途径的官能团之间， 确定这些连接性在光循环过程中如何变化。 这 将通过固态NMR，使用碳和氮 检测到二维氘交换。 从一组 模型化合物，交换光谱的细节将是 确立了习 然后，发色团/残基的变化 连通性将在光适应研究 细菌视紫红质和质子动力的光中间体 光循环

Cation pumping systems play important roles in many cellular processes such as cell growth, motility, and tumorigenesis. Despite their ubiquity and importance, the molecular mechanism for vectoral transport is not well understood. Bacteriorhodopsin provides a robust model system for cation transport. It is a membrane bound protein from Halobacterium salinarium that acts as a light-driven proton pump. From work done to date it is known that it comprises a bundle of seven alpha helices and a retinal chromophore bound by a Schiff base at Lys216. It is also know that the photocycle comprises at least five intermediates. The goal of this work is to define connectivities between functional groups involved in the transport pathway and to determine how these connectivities change during the photocycle. This will be accomplished by solid state NMR, using carbon and nitrogen detected two dimensional deuterium exchange. Starting with a set of model compounds, the details of the exchange spectra will be established. Then, the variations in the chromophore/residue connectivity will be investigated in the light adapted bacteriorhodopsin and the photo intermediates of the proton-motive photocycle.