TRANS ACTIVATED EXPRESSION OF HSV IMMEDIATE EARLY GENES

HSV 立即早期基因的反式激活表达

• 批准号: 6258866
• 负责人: Steven J. Triezenberg
• 金额: $ 0.04万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258866
• 关键词: biological products; biomedical resource; enzymes; proteins

VP16 is a herpes simplex virus protein that functions as a potent transcriptional activator of the viral immediate early genes. The transcriptional activation domain of VP16 has been widely adopted in studies of the mechanisms of transcriptional activation in eukaryotes. The overall goal of this project is to understand the structure of the VP16 activation domain, and the nature of its interactions with the host cell transcriptional machinery. VP16 is known to be a phosphoprotein when present in the virion and in infected cells; moreover, the Gal4-VP16 fusion protein frequently used in mechanistic studies is phosphorylated as a consequence of transcriptional activation. We have used MALDI-MS and peptide mass mapping as one tool in our approach to defining the phosphorylation of VP16 and of Gal4-VP16. We aim to identify the amino acid(s) that are modified, and any changes in phosphorylation that occur during the infectious cycle; mass spectrometry provides a sensitive and accurate assay.

VP 16是一种单纯疱疹病毒蛋白， 病毒立即早期基因的转录激活因子。 的 VP 16的转录激活结构域已被广泛采用， 真核生物转录激活机制的研究。 本项目的总体目标是了解 VP 16激活结构域，以及其与 宿主细胞转录机制。 VP 16被认为是一种 当存在于病毒体和感染的细胞中时的磷蛋白; 此外，Gal 4-VP 16融合蛋白经常用于机制研究， 研究表明，磷酸化是转录的结果， activation. 我们使用MALDI-MS和肽质量图谱作为一个 在我们的方法中定义VP 16的磷酸化和 Gal4-VP16。 我们的目标是鉴定被修饰的氨基酸， 以及感染过程中磷酸化的任何变化 循环;质谱法提供了灵敏和准确的测定。