TRANS ACTIVATED EXPRESSION OF HSV IMMEDIATE EARLY GENES

VP16是一种单纯疱疹病毒蛋白，其功能是 病毒即刻早期基因的转录激活物。这个 VP16的转录激活结构域已被广泛应用于 真核生物转录激活机制的研究。 本项目的总体目标是了解 VP16激活结构域，以及与VP16激活结构域相互作用的性质 宿主细胞转录机制。众所周知，VP16是一种 存在于病毒粒子和受感染细胞中的磷蛋白； 此外，Gal4-VP16融合蛋白在机械上经常使用 研究被磷酸化是转录的结果 激活。我们将MALDI-MS和多肽质量图谱结合在一起 我们定义VP16和VP16的磷酸化的方法中的工具 Gal4-VP16。我们的目标是鉴定被修饰的氨基酸(S)， 以及在感染过程中发生的任何磷酸化变化 循环；质谱学提供了灵敏和准确的分析。

VP16 is a herpes simplex virus protein that functions as a potent transcriptional activator of the viral immediate early genes. The transcriptional activation domain of VP16 has been widely adopted in studies of the mechanisms of transcriptional activation in eukaryotes. The overall goal of this project is to understand the structure of the VP16 activation domain, and the nature of its interactions with the host cell transcriptional machinery. VP16 is known to be a phosphoprotein when present in the virion and in infected cells; moreover, the Gal4-VP16 fusion protein frequently used in mechanistic studies is phosphorylated as a consequence of transcriptional activation. We have used MALDI-MS and peptide mass mapping as one tool in our approach to defining the phosphorylation of VP16 and of Gal4-VP16. We aim to identify the amino acid(s) that are modified, and any changes in phosphorylation that occur during the infectious cycle; mass spectrometry provides a sensitive and accurate assay.