Translating potent SARS-Cov-2 neutralising nanobodies from the lab to the clinic

将有效的 SARS-Cov-2 中和纳米抗体从实验室转化为临床

• 批准号: MR/V03958X/1
• 负责人: Ray Owens
• 金额: $ 25.53万
• 依托单位: Rosalind Franklin Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FV03958X%2F1
• 关键词: Translating; potent; SARS; Cov; neutralising

There is currently no cure for Covid-19. However, treatment with antibodies that block SARS-CoV2 from infecting cells may halt or ameliorate disease. We have produced very potent mini-antibodies or nanobodies that neutralise the virus in "test-tube" experiments with 100 % efficiency. The small size and stability of the nanobodies make them candidates for direct delivery into the lungs using a nebuliser device with the idea of rapidly reducing infection in the airways. The highly neutralising SARS-Cov2 nanobodies that we have generated are ready for animal tests. Successful demonstration of activity in animals will be the key step to developing this novel way of treating Covid19 with antibodies.

目前没有治愈COVID-19的方法。然而，用阻断SARS-CoV 2感染细胞的抗体治疗可能会停止或改善疾病。我们已经生产出非常有效的微型抗体或纳米抗体，在“试管”实验中以100%的效率中和病毒。纳米抗体的小尺寸和稳定性使它们成为使用雾化器装置直接递送到肺部的候选者，其想法是快速减少气道中的感染。我们已经产生的高度中和SARS-Cov 2纳米抗体已经准备好进行动物试验。在动物中成功证明活性将是开发这种用抗体治疗Covid 19的新方法的关键一步。

项目成果

Chemistry towards Biology-Instruct: Snapshot.

• DOI: 10.3390/ijms232314815
• 发表时间: 2022-11-26
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
• 影响因子: 5.6
• 作者: Hricovini, Milos;Owens, Raymond J.;Bak, Andrzej;Kozik, Violetta;Musial, Witold;Pierattelli, Roberta;Majekova, Magdalena;Rodriguez, Yoel;Musiol, Robert;Slodek, Aneta;Starha, Pavel;Pietak, Karina;Slota, Dagmara;Florkiewicz, Wioletta;Sobczak-Kupiec, Agnieszka;Jampilek, Josef
• 通讯作者: Jampilek, Josef

Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes.

• DOI: 10.1073/pnas.2205412119
• 发表时间: 2022-08-02
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1

Structural Biology of Nanobodies against the Spike Protein of SARS-CoV-2.

• DOI: 10.3390/v13112214
• 发表时间: 2021-11-03
• 期刊: Viruses
• 作者: Tang Q;Owens RJ;Naismith JH
• 通讯作者: Naismith JH

Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2

• DOI: 10.1038/s41594-020-0469-6
• 发表时间: 2020-07-13
• 期刊: NATURE STRUCTURAL & MOLECULAR BIOLOGY
• 影响因子: 16.8
• 作者: Huo, Jiangdong;Le Bas, Audrey;Naismith, James H.
• 通讯作者: Naismith, James H.