The Oxford Protein Production Facility-UK

英国牛津蛋白质生产设施

• 批准号: MR/K018779/1
• 负责人: Ray Owens
• 金额: $ 329.46万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FK018779%2F1
• 关键词: Oxford; Protein; Production; Facility; UK

Proteins are essential components of all living cells. They comprise chains of amino acids coded for by specific DNA sequences and fold into three dimensional structures, that determine their function, for example as biological catalysts. Therefore, understanding protein structure and function is key to exploiting the information generated from sequencing of the human genome and in particular those of many human pathogens. Knowledge of individual proteins has made a major contribution to the development of a number of important drugs, e.g. Zanamivir (influenza neuraminidase inhibitor), Lapatinib (kinase inhibitor), Darunavir (HIV protease inhibitor). However, high throughput DNA sequencing continues to add to the database of known proteins at a rate that far exceeds our capacity to decipher their function. Therefore, accelerating the production of recombinant proteins is key to closing the gap between the acquisition of protein sequences and the determination of their structure and function. In response to this challenge, the Oxford Protein Production Facility will provide infrastructure to underpin the study of proteins on different length and time scales from atomic resolution (x-ray crystallography) to whole cell systems (optical microscopy).

蛋白质是所有活细胞的基本组成部分。它们包含由特定DNA序列编码的氨基酸链，并折叠成三维结构，这决定了它们的功能，例如作为生物催化剂。因此，了解蛋白质的结构和功能是利用人类基因组测序产生的信息的关键，特别是许多人类病原体的基因组测序。对单个蛋白质的了解对许多重要药物的开发做出了重大贡献，例如扎那米韦（流感神经氨酸酶抑制剂）、拉帕替尼（激酶抑制剂）、达芦那韦（HIV蛋白酶抑制剂）。然而，高通量DNA测序继续增加已知蛋白质的数据库，其速度远远超过我们破译其功能的能力。因此，加速重组蛋白的生产是缩小蛋白质序列的获得与其结构和功能的确定之间的差距的关键。为了应对这一挑战，牛津蛋白质生产设施将提供基础设施，以支持从原子分辨率（X射线晶体学）到全细胞系统（光学显微镜）的不同长度和时间尺度上的蛋白质研究。

项目成果

Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.

• DOI: 10.1016/j.sbi.2013.04.003
• 发表时间: 2013-06
• 期刊: Current opinion in structural biology
• 影响因子: 6.8
• 作者: Aricescu AR;Owens RJ
• 通讯作者: Owens RJ

Crystal Structures of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport.

• DOI: 10.1016/j.str.2015.07.016
• 发表时间: 2015-10-06
• 期刊: Structure (London, England : 1993)
• 作者: Beale JH;Parker JL;Samsudin F;Barrett AL;Senan A;Bird LE;Scott D;Owens RJ;Sansom MSP;Tucker SJ;Meredith D;Fowler PW;Newstead S
• 通讯作者: Newstead S

SilE is an intrinsically disordered periplasmic "molecular sponge" involved in bacterial silver resistance.

• DOI: 10.1111/mmi.13399
• 发表时间: 2016-09
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Asiani KR;Williams H;Bird L;Jenner M;Searle MS;Hobman JL;Scott DJ;Soultanas P
• 通讯作者: Soultanas P

Diversity between mammalian tolloid proteinases: Oligomerisation and non-catalytic domains influence activity and specificity.

• DOI: 10.1038/srep21456
• 发表时间: 2016-02-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Bayley CP;Ruiz Nivia HD;Dajani R;Jowitt TA;Collins RF;Rada H;Bird LE;Baldock C
• 通讯作者: Baldock C

Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli.

• DOI: 10.3791/52357
• 发表时间: 2015-01-06
• 期刊: Journal of visualized experiments : JoVE
• 作者: Bird LE;Rada H;Verma A;Gasper R;Birch J;Jennions M;Lӧwe J;Moraes I;Owens RJ
• 通讯作者: Owens RJ