Accelerating medicine development timelines through new approaches in knowledge extraction from diverse biological data sets

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Never has the impact of the development time of a new medicine been better understood with the current situation in the UK relating to COVID. This remains and has always been a big driver for Pharmaceutical companies, to reduce the time it takes from identification of an interesting compound or vaccine to a marketed medicine. The main focus of this project is to develop new Artificial Intelligence and Machine Learning (AI/ML) analytics tools to speed up the identification of immunological medicines, including small molecules, biologics and vaccines. One of the biggest challenges we face in drug development that delays our ability to bring medicines to market quickly is the high rates of candidate molecule termination due to poor clinical efficacy and poor pre-clinical safety. To reduce the high rates of compound attrition, we are implementing disease relevant and physiological human cellular models in early stage discovery. We are using these cellular models to identify new therapeutic targets and screen our pharmacological agents to prioritise those with a better chance of success and to stop projects earlier with either efficacy or safety liabilities. These assays often rely on cellular imaging, in recent years automated microscopy has opened up the ability to characterise the state and phenotypes of cells, at single cell and even subcellular resolution. Thus, biological diversity can be visualized and the effects of perturbations on cells can be quantified more richly than by almost any other means. Relating this back to the challenge of compound failure we can use this imaging data to study compound effects in complex human in vitro systems to enable us to identify potential efficacy and safety risks much earlier in drug discovery enabling us to prioritise those medicines with a higher chance of success. As well as automated imaging, advancements have also been made to study other high content data sets such as transcriptomic, and proteomic information. This used to be completed in a screening cascade of assays all run independently to optimise our pharmacology, we are now able to multiplex these endpoints in the same system enabling us to pull together a complete fingerprint of cellular activity much faster.With these models in place, the current challenge in drug discovery is that the level of complexity of both cellular models as well as the data derived from these models is often beyond the level of data analytics technologies we have available. There is an interesting relationship between data volume and understanding. Initially if you increase the amount of information you have on a system, your understanding increases before reaching a point where understanding rapidly decreases and increased data actually causes confusion as it becomes impossible to interpret. This project is focused on resolving this bottleneck in two phases. Firstly, in recent years, AI methods, specifically convolutional deep learning methods, have revolutionised the field of computer vision by achieving performance often better than humans in image interpretation. In phase one of this project we intend to use AI methods to build a toolbox of image analytics solutions that will be used across our cellular imaging studies, to gather more information from our high-resolution images and transform millions of pixels into parameters. Similar deep learning networks have also now demonstrated the ability to explore vast data sets to interpret and deliver biological understanding. Thus, the second phase of this project will be applying AI tools to transform the millions of parameters obtained from all our high content technologies into features and mechanistic information that can be used to support project decisions.By utilising the immense power of AI approaches in image analysis and big data analytics we hope to enable a better understanding of both chemical and genetic perturbations, ultimately improving our success rate in the clinic.