PHOTON FLUORESCENCE IMAGE:CAMPTOTHECIN ANTICANCER DRUGS & CARRIER AT TUMOR SITES

我们已经开始在LAMP使用双光子显微镜来研究 FDA批准的抗癌药物喜树碱衍生物 胶质母细胞瘤细胞。为了进一步描述细胞药理学 在这类高度亲脂的喜树碱中，我们研究了 水溶物与高水溶物的差异相互作用 亲脂性喜树碱(DB67)与人胶质母细胞瘤细胞。图片 用双光子显微镜获得的胶质母细胞瘤细胞显示 药物亲脂性与药物的明确相关性 在细胞中达到的荧光水平。非常高的水平 用高度亲脂的DB67进行染色，水平下降 药物的亲脂性降低。我们的数据表明，DB67 可广泛与胶质母细胞瘤细胞等细胞相互作用 现在也在对线路进行研究。未来的研究将涉及 喜树碱与多种癌症相互作用的特征 利用双光子显微技术研究细胞和肿瘤球体 技术比较人血清白蛋白(HSA)如何调节 蛋白质相互作用药物的细胞积累，如 喜树碱与相互作用减弱的药物，如新的 实验模拟DB-67。

We have begun to use the two-photon microscope at LAMMP to study FDA approved anticancer agents, Camptothecin derivatives, in single glioblastoma cells. To further characterize the cellular pharmacology of this new class of highly lipophilic camptothecins, we have studied the differential interactions of water-soluble versus highly lipophilic camptothecins (DB67) with human glioblastoma cells. Images obtained using two-photon microscopy of glioblastoma cells show a clear correlation with between drug lipophilicity and drug fluorescence levels achieved in the cells. Very high levels of staining were achieved with highly lipophilic DB67, and levels fell off with decreasing drug lipophilicity. Our data indicate that DB67 can extensively interact with the glioblastoma cells, and other cell lines are now being studied as well. Future studies will involve characterizing camptothecin interactions with a variety of cancer cells and tumor spheroids using the two-photon micro sco py technology. It will be interesting to compare how HSA modulates the cellular accumulation of a protein-interactive drug such as camptothecin versus a drug with reduced interactions such as the new experimental analog DB-67.