CRYSTALLOGRAPHIC ANALYSIS OF FERRIC IRON BINDING PROTEIN

三价铁结合蛋白的晶体分析

• 批准号: 6119358
• 负责人: CHRISTOPHER M BRUNS
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119358
• 关键词: bacteria; biomaterials; communicable diseases

Multiwavelength anomalous dispersion (MAD) data using anomalous scattering from selenium and iron will be used to determine the structure of ferric iron binding protein (FBP) from Haemophilus influenzae. This protein is vital to several pathogenic bacteria and is a promising target of structure-based drug design. Attempts to determine the crystal structure by traditional methods have been extremely frustrating. Very strong anomalous scattering from the iron atom along with incorporated seleno-methionine will make this an ideal candidate for MAD phasing. The crystals diffract to 1.6 E resolution.

使用反常色散的多波长反常色散（MAD）数据 从硒和铁的散射将被用来确定 嗜血杆菌三价铁结合蛋白结构 流感。 这种蛋白质对几种致病菌至关重要， 是基于结构的药物设计的一个很有前途的目标。 试图 用传统方法测定晶体结构， 非常沮丧 铁的异常散射很强 一个沿着的硒代甲硫氨酸将使其成为理想的 MAD阶段的候选人。 晶体的分辨率为1.6E。