CELL CYCLE PROGRESSION IN NIJMEGEN BREAKPOINT SYNDROME IRRADIATED CELLS

奈梅亨断裂综合征(NBS)的特点是 电离辐射的敏感性和癌症风险的增加。 多参数流式细胞术，包括BrdU含量分析， 用于研究DNA损伤反应，包括细胞 在原代NBS成纤维细胞系中，周期停滞。初步研究 表明NBS细胞在对 电离辐射。在正在进行的研究中，我们会进一步研究 辐射对其他可能存在差异的NBS细胞系的影响 撕裂综合征。

Nijmegen breakage syndrome (NBS) is characterized by extreme sensitivity of ionizing radiation and increased risk for cancer. Multiparameter flow cytometry, including BrdU content analysis, is being used to investigate the DNA damage responses, including cell cycle arrest, in primary NBS fibroblast cell line. Initial studies indicate that NBS cells show normal G1 and G2 arrest in response to ionizing radiation. In on going studies we will further examine the effect of radiation on other NBS cell lines with potentially different breakage syndromes.