STRUCT OF HLA CW3 ALLOTYPE SPECIFIC KILLER CELL INHIBITORY RECEPTOR KIR2DL2

HLA CW3 同种异型特异性杀伤细胞抑制受体 KIR2DL2 的结构

• 批准号: 6205784
• 负责人: GARY SNYDER
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205784
• 关键词: biological products; biomedical resource; biotechnology; enzymes; genetics; nucleic acids; proteins; spectrometry; technology /technique

The current National and world wide genome sequencing efforts are reaching fruition in many cases. A large number of genomes from uni-cellular organisms (e.g., Mycoplasma genitalium, Haemophilius influenzae, E. coli and Saccharomyces cerevisiae) have already been sequenced and the completion of several multi-cellular genomes (e.g., Caenorhabditis elegans and Homo sapiens) is anticipated in the near future. Once the primary nucleotide sequences are accumulated, the next phase in high-throughput genome research is functional analysis and the production of functional interaction maps. These strategies are directed at identifying the function and interacting partners of each gene product, and these efforts are well underway. The logical extension and ultimate goal of high-throughput Genome Science must be the assemblage of structure-function correlates for each gene product. The realization of this goal requires a plausible structural model, which at present is not available for the vast majority of proteins. As a first step in this effort, we have selected 16 sequences from yeast to clone, express, purify, and solve their structure. The selected open reading frames are shown in table 1. The table shows the ID # for our purposes, the Swiss Protein database accession #, the yeast database #, the length of the protein, and the aa cloned based on domain analysis. The enzyme classification # (if known) and the possible function. ID Source Swiss-Prot Yeast DB Protein length aa cloned EC# Possible function P001 Yeast P06633 YOR202W 220 all 4.2.1.19 IGPD (histidine synthesis) Yeast P46969 YJL121C 238 all 5.1.3.1 ribulose phosphate epimerase P003 Yeast P40037 YER057C 129 all translation inhibitor? P004 Yeast P35691 YKL056C 167 all tumor protein homolog P005 Yeast P53295 YGR173W 368 all GTP-binding? P006 Yeast P32911 YNL244C 108 all protein translation SUI1 P007 Yeast P38197 YBL036C 257 all unknown P008 Yeast P38075 YBR035C 228 all 1.4.3.5 PNP/PMP oxidase P009 Yeast P40099 YER183C 211 all 6.3.3.2 Tetrahydrofo late related P010 Yeast P49017 YML110C 307 all 2.1.1.-ubiquinone methyltransf er P011 Yeast P12904 YGL115W 322 all protein kinase gamma chain P012 Yeast P52286 YDR328C 193 all centromere binding CBF3 D P013 Yeast P53878 YNL181W 407 all 1.1.1.15 9 steroid oxidoreducta se P014 Yeast YOR221C 384 124-368 2.3.1.39 acyl carrier transacylase P015 Yeast YCR033W 1226 885-935 C-MYB DNA-binding P016 Yeast YPL217C 1183 63-182 EF-TU GTP binding

目前国家和世界范围的基因组测序工作是 在很多情况下都能开花结果。 大量的基因组来自 单细胞生物（例如，生殖支原体，血友病 influenzae、E.大肠杆菌和酿酒酵母）已经被 测序并完成几个多细胞基因组（例如， 秀丽隐杆线虫和智人）预计在不久的 未来 一旦积累了一级核苷酸序列， 高通量基因组研究的下一个阶段是功能分析 以及功能交互图的制作。 这些策略 目的是确定功能和相互作用的伙伴， 每一个基因产物，这些努力正在顺利进行。 逻辑 高通量基因组科学的延伸和最终目标必须是 每个基因产物的结构-功能相关性的集合。 这一目标的实现需要一个合理的结构模型， 目前绝大多数蛋白质都不能获得这种蛋白质。 作为这项工作的第一步，我们选择了16个序列， 酵母进行克隆、表达、纯化和结构解析。 的 表1中示出了所选择的开放阅读帧。 该表显示 用于我们目的的ID #、Swiss Protein数据库登录号、 酵母数据库#、蛋白质的长度和基于克隆的aa 领域分析。 酶分类号（如果已知）和 可能的功能。 ID来源Swiss-Prot Yeast DB蛋白长度aa 克隆EC#可能的功能P001酵母P06633 YOR 202 W 220全部 4.2.1.19 IGPD（组氨酸合成）酵母P46969 YJL 121 C 238全部 5.1.3.1磷酸核酮糖差向异构酶P003酵母P40037 YER 057 C 129 all 翻译抑制剂 P004酵母P35691 YKL 056 C 167所有肿瘤 蛋白质同源物P005酵母P53295 YGR 173 W 368所有GTP结合？ P006 酵母P32911 YNL 244 C 108所有蛋白翻译SUI 1 P007酵母 P38197 YBL 036 C 257全部未知P008酵母P38075 YBR 035 C 228全部 1.4.3.5 PNP/PMP氧化酶P009酵母P40099 YER 183 C 211全部6.3.3.2 四氢叶酸相关P010酵母P49017 YML 110 C 307全部 2.1.1.-泛醌甲基转移酶P011酵母P12904 YGL 115 W 322全部 蛋白激酶γ链P012酵母P52286 YDR 328 C 193全部 着丝粒结合CBF 3 D P013酵母P53878 YNL 181 W 407 all 1.1.1.15 9 类固醇氧化还原酶se P014酵母YOR 221 C 384 124-368 2.3.1.39酰基 载体转酰酶P015酵母YCR 033 W 1226 885-935 C-MYB DNA结合 P016酵母YPL 217 C 1183 63-182 EF-TU GTP结合