CHARACTERIZATION OF ESTROGEN BINDING PROTEIN FROM CANDIDA ALBICANS

白色念珠菌雌激素结合蛋白的表征

• 批准号: 6456796
• 负责人: SUSAN M MILLER
• 金额: $ 27.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6456796
• 关键词: biological products; biomedical resource; drug /agent; endocrine gland /system; hormones

We are continuing studies of the estrogen binding protein from Candida albicans to establish the in vivo function of this redox enzyme. As one approach we are studying the properties of mutants of active site residues to identify those with altered catalytic and/or ligand binding properties that may be useful in trapping in vivo substrates. The Computer Graphics Laboratory is critical in helping us to visualize and generate a homology model of the enzyme based on the structure of a highly homologous enzyme from Saccharomyces cerevisiae in order to generate the mutants of interest. In addition, we are using the graphics to try to interpret observed differences in the behavior of the mutant and wild type enzymes, e.g., pH dependent behavior, in the context of residues near the active site.

我们正在继续研究雌激素结合蛋白， 白色念珠菌，以建立这种氧化还原的体内功能 酵素 作为一种方法，我们正在研究突变体的性质， 活性位点残基，以鉴定具有改变的催化和/或 可用于体内捕获的配体结合性质 印刷受体. 计算机图形实验室在帮助 我们可视化并生成基于以下的酶的同源性模型： 一种高度同源酵母酶结构 酿酒酵母以产生感兴趣的突变体。 此外，本发明还提供了一种方法， 我们用图表来解释观察到的差异， 突变体和野生型酶的行为，例如，pH依赖性 行为，在活性位点附近的残基的背景下。