PHYSIOLOGICAL BASIS FOR WEED COMPETITION IN N2 FIXING LEGUMINOUS CROP SYSTEMS

氮固豆科作物系统中杂草竞争的生理基础

• 批准号: 6220739
• 负责人: JANET MACFALL
• 金额: $ 0.38万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220739
• 关键词: Mammalia; biomedical resource; cardiovascular system; growth /development; human tissue; nuclear magnetic resonance spectroscopy

Mouse embryos between 8.5 and 19.5 days of gestation will be investigated on the 9.4 Tesla system. We will be testing pre-processing techniques of the embryos to determine what fixation techniques and what reaction mixes provide the most useful information for each embryo stage. Specifically, we will determine whether permeabalization and reaction with ferric and ferrous salts contribute to enhanced contrast of neural crest structures in 11.5 to 14.5 day mouse embryos. We will also search for the source of enhanced signal in developing bone as demonstrated in embryos between 14.5 days and birth. We will also scan embryos which have been perfused with Gd-BSA-DTPA to complete our time-series study of cardiovascular development in the normal embryo.

怀孕8.5至19.5天的小鼠胚胎将是 已研究9.4特斯拉系统。我们将测试 胚胎的前处理技术，以确定什么固定 技术和什么反应混合物提供了最有用的信息 对于每个胚胎阶段。具体来说，我们将确定是否 与铁和亚铁盐的渗透和反应有助于 在11.5~14.5天内对神经脊结构进行增强对比 小鼠胚胎。我们还将寻找增强信号的来源 在14.5天和14.5天之间的胚胎中证明了在骨骼发育中 出生。我们还将扫描已经灌输了 Gd-BSA-DTPA将完成我们的心血管时间序列研究 在正常胚胎中的发育。