ENERGETICS OF SMALL RNA PSEUDOKNOT

假结是一种主要的结构图案， RNA折叠成相当紧凑的形式。 伪结也是 控制某些mRNA的重要信号。 在 莫洛尼鼠白血病病毒，一个假结是已知的重要 在调节mRNA通读的程度，因此建立了 产生的gag和gag-pol蛋白的量。 的机理 通读事件未知。 一般来说，只有少数 不完整的研究折叠的假结，这项研究的目的是 是一个相对完整的热力学描述的折叠 一个伪结的能量学 量热数据是 补充了已经掌握的紫外线增色数据。 的 这两个数据集的组合将有助于确定 解折叠RNA 是 设想这些数据将有助于确定 的readthrough。

Pseudoknots are one of the major structural motifs which allow RNAs to fold into rather compact forms. Pseudoknots are also important signals governing the regulation of certain mRNAs. In Moloney Murine Leukemia virus, a pseudoknot is known to be important in regulating the degree of mRNA readthrough, hence establishing the amount of gag and gag-pol protein being made. The mechanism of the readthrough event is unknown. In general, there are only a few incomplete studies on the folding of pseudoknots, and this study aims to be a relatively complete thermodynamic description of the folding energetics of a pseudoknot. The calorimetry data is an essential complement to the UV hyperchromcity data already in hand. The combination of these two data sets will aid in determining the unfolding of this RNA. It is envisioned that such data will be useful in determining the mechansim of readthrough.