FLUORIMETRIC CHARACTERIZATION OF RETROVIRAL PEPTIDES: HIV

逆转录病毒肽的荧光表征：HIV

• 批准号: 6122889
• 负责人: JOSE CASAS-FINET
• 金额: $ 4.23万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122889
• 关键词: biological products; biomaterials; biomedical resource; informatics; musculoskeletal system; proteins; spectrometry

Troponin complex (troponin T (TnT), troponin I (TnI), troponin C (TnC)) plays an important role in the Ca2+ -dependent regulation of muscle contraction. To study the proximity relationships of the troponin T-I-C subunits, a series of additional mutation of TnT and 25 K fragment of TnT have been made, expressed in E.coli and purified. The single-Tap mutant K fragment of TnT (TnT/25 K W265F) was needed as the donor for distance measurements by fluorescence energy transfer. Series of donor-acceptor pairs were chosen and the spectroscopic properties measured under different buffer conditions. The distances between Tap-212 of TnT/K to Tin-Cys-96 (N96C), Tin-Cys-117 (S117C), TnC-Met-25 and TnC-Cys-98 were measured. The distance measurements are being used to develop models for muscle contraction.

肌钙蛋白复合体(肌钙蛋白T(TnT)、肌钙蛋白I(TnI)、肌钙蛋白C (TNC))在钙依赖的调节中发挥重要作用 肌肉收缩。要研究对象之间的邻近关系 肌钙蛋白T-I-C亚基，TnT和25的一系列额外突变 制备了TnT的K片段，并在大肠杆菌中进行了表达和纯化。 TNT(TNT/25K W265F)的单次敲击突变K片段需要 通过荧光能量转移进行距离测量的供体。 选择了一系列的供体-受体对，并进行了光谱分析 在不同缓冲条件下测量的特性。距离 在TNT/K的Tap-212至Tin-Cys-96(N96C)、Tin-Cys-117(S117C)之间， 测定TNC-Met-25和TNC-Cys-98。距离测量 被用来开发肌肉收缩的模型。