Leica TCS SP5 laser scanning confocal microscope

描述（由申请人提供）：Linser, Paul J.确保惠特尼实验室显微镜能力的连续性佛罗里达大学惠特尼实验室是一个致力于细胞和分子生物学的研究机构。常驻教员研究细胞生物学中广泛的模型系统和基本问题。该研究所大多数研究的自然组成部分是使用先进的显微技术分析细胞功能和细胞功能定位。激光扫描共聚焦显微镜已成为这些分析中许多方面不可或缺的工具。惠特尼实验室的老化（7年）徕卡LSCM SP2不再支持制造新零件，这导致停机时间越来越长。此外，SP2的原始设计在当前研究环境中的局限性给惠特尼实验室的许多具体研究带来了技术障碍。这项拨款提案是为共享仪器拨款（SIG）提供资金，用于购买新的，最先进的徕卡TCS SP5共聚焦显微镜。在过去的七年中，这项技术在硬件和软件方面都取得了重大进展，这为许多新的研究提供了机会。与过时的SP2相关的频繁故障相结合，更换显微镜是惠特尼实验室研究所长期成就的持续成功不可或缺的一部分。公共卫生相关性：本提案为惠特尼实验室的一台徕卡TCS SP5激光扫描共聚焦显微镜提供资金。惠特尼实验室是一个尖端的生物医学实验室，30多年来，在基础细胞生物学、神经科学和公共卫生等研究领域提供了许多突破性的出版物。技术同步（例如共聚焦显微镜）对于佛罗里达大学惠特尼实验室继续对生物医学做出贡献至关重要。

DESCRIPTION (provided by applicant): Linser, Paul J. Ensuring the Continuity of the Whitney Laboratory's Microscopy Capacity The Whitney Laboratory of the University of Florida is a research Institute dedicated to cell and molecular biology. The resident faculty members investigate a wide range of model systems and fundamental questions in cell biology. A natural part of most investigations at the Institute is analysis of cell function and cell functionality localization using advanced microscopic techniques. A laser scanning confocal microscope has become an integral and indispensable tool in many aspects of these analyses. The Whitney Lab's aging (7 year old) Leica LSCM SP2 is no longer supported with the manufacture of new parts which has lead to increasingly lengthy periods of downtime. Furthermore, the limitations on the original design of the SP2 in the current research climate creates technical hurdles to many specific investigations at the Whitney Lab. This grant proposal is for a Shared Instrumentation Grant (SIG) to provide funds for the purchase of a new, state-of-the-art Leica TCS SP5 confocal microscope. Major advances have occurred in both hardware and software in the past seven years for this technology which opens many new research opportunities. Paired with the frequent periods of malfunction associated with the out-dated SP2, a replacement microscope is integral to the continued success of the Whitney Lab Research Institute's long history of accomplishment. PUBLIC HEALTH RELEVANCE: This proposal is to provide funding for a Leica TCS SP5 laser scanning confocal microscope at the Whitney Laboratory. The Whitney Lab is a cutting edge Biomedical Laboratory and for over 30 years has provided numerous breakthrough publications in areas of research including fundamental cell biology, neuroscience and public health. Technical concurrency (e.g. confocal microscope) is crucial to continued contributions to biomedicine made by the Whitney Laboratory of the University of Florida.