Leica TCS SP5 laser scanning confocal microscope

Leica TCS SP5 激光扫描共焦显微镜

• 批准号: 7591498
• 负责人: PAUL J LINSER
• 金额: $ 49.98万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591498
• 关键词: 7 year old; Applications Grants; Area; Arts; Biological Models; Cell physiology; Cells; Cellular biology; Climate; Computer software; Ensure; Faculty; Florida; Funding; Grant; Institutes; Investigation; Laboratories; Lasers; Lead; Microscope; Microscopic; Microscopy; Molecular and Cellular Biology; Neurosciences; Public Health; Publications; Recording of previous events; Research; Research Institute; Scanning; Techniques; Technology; Universities; design; instrumentation; member; public health relevance; success; tool

DESCRIPTION (provided by applicant): Linser, Paul J. Ensuring the Continuity of the Whitney Laboratory's Microscopy Capacity The Whitney Laboratory of the University of Florida is a research Institute dedicated to cell and molecular biology. The resident faculty members investigate a wide range of model systems and fundamental questions in cell biology. A natural part of most investigations at the Institute is analysis of cell function and cell functionality localization using advanced microscopic techniques. A laser scanning confocal microscope has become an integral and indispensable tool in many aspects of these analyses. The Whitney Lab's aging (7 year old) Leica LSCM SP2 is no longer supported with the manufacture of new parts which has lead to increasingly lengthy periods of downtime. Furthermore, the limitations on the original design of the SP2 in the current research climate creates technical hurdles to many specific investigations at the Whitney Lab. This grant proposal is for a Shared Instrumentation Grant (SIG) to provide funds for the purchase of a new, state-of-the-art Leica TCS SP5 confocal microscope. Major advances have occurred in both hardware and software in the past seven years for this technology which opens many new research opportunities. Paired with the frequent periods of malfunction associated with the out-dated SP2, a replacement microscope is integral to the continued success of the Whitney Lab Research Institute's long history of accomplishment. PUBLIC HEALTH RELEVANCE: This proposal is to provide funding for a Leica TCS SP5 laser scanning confocal microscope at the Whitney Laboratory. The Whitney Lab is a cutting edge Biomedical Laboratory and for over 30 years has provided numerous breakthrough publications in areas of research including fundamental cell biology, neuroscience and public health. Technical concurrency (e.g. confocal microscope) is crucial to continued contributions to biomedicine made by the Whitney Laboratory of the University of Florida.

描述（由申请人提供）：Linser，Paul J.确保惠特尼实验室显微镜能力的连续性佛罗里达大学的惠特尼实验室是一家专门针对细胞和分子生物学的研究所。居民教师研究了各种模型系统和细胞生物学中的基本问题。研究所大多数研究的自然部分是使用高级微观技术分析细胞功能和细胞功能定位。在这些分析的许多方面，激光扫描共聚焦显微镜已成为一种不可或缺的工具。惠特尼实验室（Whitney Lab）的衰老（7岁）Leica LSCM SP2不再支持制造新零件，这导致停机时间越来越长。此外，当前研究氛围中SP2的原始设计的局限性为惠特尼实验室的许多特定研究带来了技术障碍。该赠款提案是针对共享仪器赠款（SIG）提供的，用于购买新的，最先进的Leica TCS SP5共聚焦显微镜。在过去的七年中，硬件和软件在这项技术中都取得了重大进展，这为许多新的研究机会。与临时SP2相关的频繁发生故障时期，替换显微镜是惠特尼实验室研究所持续成功的悠久成就历史的持续成功不可或缺的一部分。公共卫生相关性：该建议是为惠特尼实验室的Leica TCS SP5激光扫描共聚焦显微镜提供资金。惠特尼实验室（Whitney Lab）是一家尖端生物医学实验室，已有30多年的历史在研究领域提供了许多突破性的出版物，包括基本细胞生物学，神经科学和公共卫生。技术并发（例如共聚焦显微镜）对于佛罗里达大学惠特尼实验室的生物医学持续贡献至关重要。