MODELING DNA DIVERSITY IN CARDIOVASCULAR HEALTH/DISEASE

描述（改编自申请人摘要）项目1是一项研究 合作研究计划的一部分，涉及两个其他研究 组件，项目2，韦斯，PI和项目3，Sing，PI。 人类生物学中最复杂和最具挑战性的问题之一， 医学界正在定义DNA序列变异与 复杂疾病定量危险因素的个体间变异 具有多因素病因的。 因为他们对人类基本的知识 DNA序列的增加，所以将他们的需要，以确定范围的自然 人类群体中的变异，并探讨 核苷酸多样性和表型变异在人类健康的措施。 项目1的目标是识别和测量DNA序列变异， 13个基因在参与的关键生理功能中发挥核心作用， 心血管疾病（CVD）的发展，即参与 脂质代谢 项目1将采用最先进的自动化 基于荧光的测序和高通量DNA基因分型方法 发现并评估三个人群的DNA序列变异： 来自明尼苏达州罗切斯特的非西班牙裔白人（低CVD风险），非裔美国人 来自杰克逊、MS（中等CVD风险）和来自北卡罗来纳州的非西班牙裔白人 卡累利阿，芬兰（高心血管疾病风险）。 这些研究将提供一个 前所未有

DESCRIPTION (adapted from the applicants abstract) Project 1 is a research component of a Cooperative Research Program involving two other research components, Project 2, Weiss, PI and Project 3, Sing, PI. One of the most complex and challenging problems in human biology and medicine is defining the relationship between DNA sequence variation and interindividual variation in quantitative risk factors for complex diseases having a multifactorial etiology. As their knowledge about the basic human DNA sequence increases, so will their need to define the range of natural variation n human populations and to explore the relationship between nucleotide diversity and phenotype variation in measures of human health. The goal of Project 1 is to identify and measure DNA sequence variation in 13 genes that play a central role in key physiological functions involved in the development of cardiovascular disease (CVD), i.e. genes involved in lipid metabolism. Project 1 will apply state-of-the-art automated fluorescence-based sequencing and high-throughput DNA genotyping methods to uncover and assess DNA sequence variation in three human populations: non-Hispanic Whites from Rochester, MN (low CVD risk), African-Americans from Jackson, MS (intermediate CVD risk) and non-Hispanic Whites from North Karelia, Finland (high CVD risk). These studies will provide an unprecedented