DEVELOPMENTAL MORPHOGENETICS OF THE MOUSE DENTITION

哺乳动物的牙列是一个复杂的器官，其正常发育是 对健康有重大意义，但其发育不全和恶化 严重不足的研究。 牙列也是一种重要的遗传学模式系统 复杂的、分段的器官，通过组织间的相互作用而发育。 这项研究启动项目将研究调控基因。 小鼠牙胚中的表达模式。首先，从10.5获得了mrna。 将通过聚合酶链式反应(PCR)方法对日牙病菌进行筛选 扩增所有表达的同源异型(Hox)基因。扩增的产物将 进行测序和鉴定；以前未鉴定的基因将被克隆 并使用小鼠基因组文库绘制了图谱。将使用类似的程序 对于Int-2、I型胶原和Tenascin(以及密切同源的基因)， 参与控制细胞分化和细胞外 矩阵制作。 将在不同的牙胚切片上进行原位杂交 发育年龄和每颗牙齿的形态截然不同的部分 为确定的基因开发时空调控表达图谱 上面。最后，将开展工作来开发基于聚合酶链式反应的方法 提高了显微组织学表达分析的灵敏度。 本研究将为系统的研究奠定基础。 细胞-细胞相互作用、神经峰函数和细胞形态发生学 哺乳动物牙齿，直接应用于人类牙列和 牙齿发育不全。 这是对NIDR的小额赠款计划的回应，作为研究再培训 肯尼斯·M·韦斯的倡议，并将正在进行的方法扩展到 弗兰克·H·拉德尔的新领域。

The mammalian dentition is a complex organ whose normal development is of major health importance, yet whose dysgenesis and deterioration have been grossly under-studied. The dentition is also an important model system for the genetics of complex, segmented organs that develop through tissue interactions. This research-initiation project will investigate regulatory gene expression patterns in mouse tooth germs. First, mRNA obtained from 10. 5 daytooth germs will be screened by polymerase chain reaction (PCR) methods to amplify all expressed homeotic (Hox) genes. The amplified products will be sequenced and identified; previously unidentified genes will be cloned and mapped using a mouse genome library. Similar procedures will be used for Int-2, Collagen I, and tenascin (and closely homologous genes), that are involved in the control of cell differentiation and extra-cellular matrix production. In situ hybridization will be done on sections of tooth germs at various developmental ages and in morphologically distinct parts of each tooth, to develop a spatio-temporal regulatory expression map for genes identified as above. Finally, work will be done to develop PCR-based methods for improved sensitivity in microhistological expression analysis. This study will lay the ground work for systematic investigations of cell-cell interaction, neural crest function, and the morphogenetics of mammalian teeth, with direct application to the human dentition and to dental dysgenesis. This is responsive to NIDR's Small Grants Program, as a research retraining initiative for Kenneth M. Weiss, and an extension of ongoing methods to a new area for Frank H. Ruddle.