GENES IN TEETH

牙齿中的基因

• 批准号: 2131799
• 负责人: KENNETH M. WEISS
• 金额: $ 15.91万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2131799
• 关键词: antisense nucleic acid; complementary DNA; dental development; developmental genetics; embryo /fetus tissue /cell culture; gene expression; genetic library; genetic mapping; genetic regulatory element; homeobox genes; in situ hybridization; laboratory mouse; nucleic acid sequence; organ culture; polymerase chain reaction; tooth

DESCRIPTION: (Adapted from Applicant's Abstract) The dentition is a segmented organ whose evolutionary history and morphology strongly resemble other segmental systems like the vertebral column or limb. However, teeth use different genes, and perhaps different mechanisms, to attain that end. This is an effort, with four related components, of gene identification, expression, function and characterization for the homeobox developmental genes. In Specific Aim 1, classes of developmental genes from mouse tooth germ cDNA will be isolated and screened to identify regulatory genes, especially members of the homeobox gene family and related genes, that are expressed specifically in teeth or only in incisors or molars. This family is known to be involved in dental development. Mid-gestation incisor and molar cDNA libraries will also be made. In Specific Aim 2 expression maps of Dlx-5, a distal-less class homeobox gene identified in developing mouse teeth, and closely related developmental genes expressed in teeth, will be characterized by in situ hybridization of antisense probes to developing tooth germs across gestation, looking for timed expression patterns of these genes within and between teeth. Specific Aim 3 proposes to explore the function of Dlx-5 relative to its expression map. Dlx-5 expression will be inactivated by exposing embryonic teeth cultured in vitro to antisense RNA to the gene. Specific Aim 4 proposes to clone and sequence Dlx-5 to identify its structure and chromosomal map location. If expression and function studies confirm its importance in tooth development, the intergenic and regulatory regions of Dlx- 5 will be sequenced, and closely linked genes identified. The systematic approach to dental morphogenetics outlined in this application is important because combinatorial gene expression is probably the key to this process.

描述：(改编自申请者摘要)该牙列是 具有强烈的进化史和形态的节状器官 类似于其他节段性系统，如脊柱或四肢。 然而，牙齿使用不同的基因，也许还有不同的机制， 以达到这一目的。这是一项努力，包括四个相关的组成部分， 基因的识别、表达、功能和特性 同源盒发育基因。在具体目标1中，分类 将从小鼠牙胚基因中分离出发育基因 筛选以识别调控基因，特别是同源盒的成员 在牙齿中特异表达的基因家族和相关基因 或者只出现在门牙或磨牙上。众所周知，这个家庭参与了 牙齿发育。孕中期切牙和磨牙cdna文库将 也会被制造出来。DLX-5基因的特异靶2表达图谱 在发育中的小鼠牙齿中发现了类同源框基因，并与 在牙齿中表达的相关发育基因，将被表征 牙胚发育过程中反义探针的原位杂交 在怀孕期间，寻找这些基因的定时表达模式 牙齿内部和牙齿之间。具体目标3建议探索 DLX-5的功能与其表达谱有关。DLX-5表达式将 将体外培养的胚胎牙齿暴露于 该基因的反义RNA。具体目标4提出克隆和测序 DLX-5用于鉴定其结构和染色体图谱定位。如果 表达和功能研究证实其在牙齿中的重要性 发展，DLX-5的基因间隔区和调节区将是 对已确定的紧密相连的基因进行了测序。系统化的方法 对于本申请中概述的牙齿形态遗传学是重要的 因为组合基因表达可能是这一点的关键 进程。