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GENES IN TEETH

牙齿中的基因

基本信息

批准号：
2131801
负责人：
KENNETH M. WEISS
金额：
$ 14.5万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-08-01 至 1998-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2131801
关键词：
antisense nucleic acid complementary DNA dental development developmental genetics embryo /fetus tissue /cell culture gene expression genetic library genetic mapping genetic regulatory element homeobox genes in situ hybridization laboratory mouse nucleic acid sequence organ culture polymerase chain reaction tooth

项目摘要

DESCRIPTION: (Adapted from Applicant's Abstract) The dentition is a segmented organ whose evolutionary history and morphology strongly resemble other segmental systems like the vertebral column or limb. However, teeth use different genes, and perhaps different mechanisms, to attain that end. This is an effort, with four related components, of gene identification, expression, function and characterization for the homeobox developmental genes. In Specific Aim 1, classes of developmental genes from mouse tooth germ cDNA will be isolated and screened to identify regulatory genes, especially members of the homeobox gene family and related genes, that are expressed specifically in teeth or only in incisors or molars. This family is known to be involved in dental development. Mid-gestation incisor and molar cDNA libraries will also be made. In Specific Aim 2 expression maps of Dlx-5, a distal-less class homeobox gene identified in developing mouse teeth, and closely related developmental genes expressed in teeth, will be characterized by in situ hybridization of antisense probes to developing tooth germs across gestation, looking for timed expression patterns of these genes within and between teeth. Specific Aim 3 proposes to explore the function of Dlx-5 relative to its expression map. Dlx-5 expression will be inactivated by exposing embryonic teeth cultured in vitro to antisense RNA to the gene. Specific Aim 4 proposes to clone and sequence Dlx-5 to identify its structure and chromosomal map location. If expression and function studies confirm its importance in tooth development, the intergenic and regulatory regions of Dlx- 5 will be sequenced, and closely linked genes identified. The systematic approach to dental morphogenetics outlined in this application is important because combinatorial gene expression is probably the key to this process.

描述：（改编自申请人摘要）该牙列是一种

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Numerous members of the Sox family of HMG box-containing genes are expressed in developing mouse teeth.

含 HMG 盒基因的 Sox 家族的许多成员在发育中的小鼠牙齿中表达。

DOI：
10.1006/geno.1996.0548
发表时间：
1996
期刊：
Genomics.
影响因子：
0
作者：
Stock,DW;Buchanan,AV;Zhao,Z;Weiss,KM
通讯作者：
Weiss,KM

Perspectives on genetic aspects of dental patterning.

关于牙齿图案遗传方面的观点。

DOI：
10.1111/j.1600-0722.1998.tb02154.x
发表时间：
1998
期刊：
European journal of oral sciences.
影响因子：
0
作者：
Weiss,K;Stock,D;Zhao,Z;Buchanan,A;Ruddle,F;Shashikant,C
通讯作者：
Shashikant,C

Patterning of the mammalian dentition in development and evolution.

哺乳动物牙列在发育和进化过程中的模式。

DOI：
10.1002/bies.950190607
发表时间：
1997
期刊：
BioEssays : news and reviews in molecular, cellular and developmental biology.
影响因子：
0
作者：
Stock,DW;Weiss,KM;Zhao,Z
通讯作者：
Zhao,Z

Dynamic interactions and the evolutionary genetics of dental patterning.