DEVELOPMENTAL MORPHOGENETICS OF THE MOUSE DENTITION

小鼠牙列的发育形态学

• 批准号: 3425693
• 负责人: KENNETH M. WEISS
• 金额: $ 4.9万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425693
• 关键词: ameloblasts; cell cell interaction; cell differentiation; collagen; complementary DNA; computer graphics /printing; dental development; dental structure; developmental genetics; embryo /fetus tissue /cell culture; enamel organ; extracellular matrix; gene expression; genetic library; histochemistry /cytochemistry; homeobox genes; in situ hybridization; laboratory mouse; longitudinal animal study; mammalian embryology; messenger RNA; molecular cloning; neural crest; northern blottings; odontoblasts; polymerase chain reaction; regulatory gene; structural genes; tissue /cell preparation; tooth

The mammalian dentition is a complex organ whose normal development is of major health importance, yet whose dysgenesis and deterioration have been grossly under-studied. The dentition is also an important model system for the genetics of complex, segmented organs that develop through tissue interactions. This research-initiation project will investigate regulatory gene expression patterns in mouse tooth germs. First, mRNA obtained from 10. 5 daytooth germs will be screened by polymerase chain reaction (PCR) methods to amplify all expressed homeotic (Hox) genes. The amplified products will be sequenced and identified; previously unidentified genes will be cloned and mapped using a mouse genome library. Similar procedures will be used for Int-2, Collagen I, and tenascin (and closely homologous genes), that are involved in the control of cell differentiation and extra-cellular matrix production. In situ hybridization will be done on sections of tooth germs at various developmental ages and in morphologically distinct parts of each tooth, to develop a spatio-temporal regulatory expression map for genes identified as above. Finally, work will be done to develop PCR-based methods for improved sensitivity in microhistological expression analysis. This study will lay the ground work for systematic investigations of cell-cell interaction, neural crest function, and the morphogenetics of mammalian teeth, with direct application to the human dentition and to dental dysgenesis. This is responsive to NIDR's Small Grants Program, as a research retraining initiative for Kenneth M. Weiss, and an extension of ongoing methods to a new area for Frank H. Ruddle.

哺乳动物的牙列是一个复杂的器官，其正常发育是由 主要健康的重要性，但其发育不良和恶化一直是 被严重忽视了 牙列也是遗传学的一个重要模型系统， 通过组织相互作用发育的复杂的、分段的器官。 本研究将探讨调控基因 小鼠牙胚中的表达模式。 首先，从10. 5 采用聚合酶链反应（PCR）方法筛选白牙胚 扩增所有表达的同源异型（Hox）基因。 扩增产物将 测序和鉴定;以前未鉴定的基因将被克隆 并使用小鼠基因组文库绘制。 将采用类似的程序 对于Int-2、胶原蛋白I和腱生蛋白（以及密切同源的基因）， 参与控制细胞分化和细胞外 矩阵生产 原位杂交将在不同的牙胚切片上进行， 发育年龄和每个牙齿的形态不同的部分， 为鉴定为以下的基因开发时空调控表达图谱： 以上 最后，将开展工作，开发基于PCR的方法， 提高了显微组织学表达分析的灵敏度。 这项研究将为系统地调查 细胞-细胞相互作用，神经嵴功能，以及 哺乳动物牙齿，直接应用于人类牙列， 牙齿发育不全 这是响应NIDR的小额赠款计划，作为一个研究再培训 Kenneth M.韦斯，并扩展正在进行的方法， 弗兰克·H的新领域拉德尔