STOPPED-FLOW OPTICAL ROTATARY DISPERSION SPECTROMETER

停流光学旋转色散光谱仪

• 批准号: 6016628
• 负责人: DANIEL B KIM-SHAPIRO
• 金额: $ 13.09万
• 依托单位: ON-LINE INSTRUMENT SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6016628
• 关键词: biomedical equipment development; circular dichroism; optical rotation; protein folding; spectrometry; stop flow technique; technology /technique development

DESCRIPTION: (Adapted from the applicant's abstract) The broad, long-range goal of OLIS is to develop instrumentation that will help research efforts in the biomedical sciences. In particular, here the applicants propose to develop the first wavelength scanning, stopped-flow instrument capable of measuring optical rotary dispersion (ORD). Presently there is no commercially available instrument capable of making time resolved, spectral circular dichroism (CD) or ORD measurements. Wavelength scanning is important in deciphering complex mechanisms. CD gives more information about certain aspects of bio-molecular structure than absorption. ORD can provide the same information as CD but with less noise and better time resolution. Spectral, time-resolved ORD measurements will provide researchers with valuable information not currently available by other means. In particular, new information related to the protein folding problem would be attainable. Understanding how a protein folds is important for biotechnological purposes (so that protein function can be designed) as well as in helping to cure diseases where mis-folded proteins may play a role (eg spongioform encephalopathies, Huntigton's disease and Alzheimer's disease). An ORD attachment will be built for their rapid scanning absorption spectrophotometer that makes 1000 spectral measurements every second. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：（改编自申请人的摘要）广泛的，长期的目标 OLIS的目的是开发仪器，这将有助于研究工作， 生物医学科学特别地，在此申请人提出开发 第一波长扫描、可测量光学的停流仪器 旋转分散（ORD）。目前还没有市售的 能够进行时间分辨的光谱圆二色性（CD）或 测量范围。波长扫描在破译复杂的 机制等CD提供了更多关于生物分子生物学某些方面的信息。 结构比吸收。ORD可以提供与CD相同的信息，但 噪声更小，时间分辨率更高。光谱、时间分辨ORD测量 将为研究人员提供目前无法获得的有价值的信息 其他方式特别是与蛋白质折叠有关的新信息 问题是可以实现的。了解蛋白质如何折叠对于 生物技术目的（以便可以设计蛋白质功能）以及 在帮助治疗疾病，其中错误折叠的蛋白质可能发挥作用（如 海绵状脑病、亨氏病和阿尔茨海默病）。一个 ORD附件将建立他们的快速扫描吸收 分光光度计每秒进行1000次光谱测量。 拟议商业应用：不可用