KINETIC STUDIES OF INDIUM LABELED LEUKOCYTES

铟标记白细胞的动力学研究

• 批准号: 6161484
• 负责人: S F LEITMAN
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161484
• 关键词: Zollinger Ellison syndrome; arteritis; cell migration; chronic granulomatous disease; clinical research; diagnosis design /evaluation; human subject; indium; leukocytes; melanoma; osteomyelitis; radiodiagnosis; radiotracer; scintillation cameras; scintillation counter

The kinetic patterns of fresh, frozen-thawed, or cultured human leukocytes are studied by tagging the cells ex vivo with 111 - Indium, a radio- isotopic label, and measuring their distribution throughout the body by means of gamma camera imaging and gamma counting of blood samples. This type of study has widespread applications. The most common use of these radio-labeled leukocyte trafficking studies is for abscess localization in cases of suspected infection not definitively diagnosed by other, non- invasive, studies. In these cases autologous or allogeneic granulocytes, collected by simple phlebotomy or by apheresis, are labeled with 50 muCi of 11 l-Indium per kg of patient weight. Fourteen studies for the purpose of abscess localization were performed in Clinical Center patients enrolled in a variety of protocols in the past 6 years. Two of these 14 were positive and led to appropriate therapy for a case of Salmonella osteomyelitis in a hemophiliac patient with HIV infection, and resection of a diverticular abscess in a patient with Zollinger-Ellison syndrome. In many of the negative studies, unnecessary surgical exploration was avoided. Indium-labeled leukocyte trafficking studies have also been investigated as diagnostic tools is-detecting large vessel inflammation in patients with Takayasu's arteritis. A prospective study performed in collaboration with NIAID and NMD/CC showed that radio-labeled autologous mononuclear cell trafficking could not accurately predict whether symptomatic patients with fever and high sedimentation rates had active arteritis, and thus could not be used to determine whether intervention with anti-inflammatory agents should be initiated. Collaborative trials with the Surgery Branch, NCI, have investigated the diagnostic utility and prognostic application of radio-labeled autologous tumor-infiltrating lymphocyte (TIL) studies in patients with metastatic melanoma. TIL trafficking studies revealed metastatic deposits that were undetected clinically, and TIL trafficking to sites of tumor was strongly correlated with tumor regression in response to infusions of TIL cells and cyclophosphamide. Most recently, radio-labeled allogeneic granulocyte trafficking studies have been used to determine whether pulmonary infiltrates in patients with chronic granulomatous disease of childhood (COD) are due to infection or to lung scarring and granuloma formation. Trafficking studies in COD patients with HLA-alloimmunization have been used to predict whether allogeneic cells will survive following transfusion and thus whether a therapeutic trial of granulocyte transfusions is warranted.

新鲜、冻融或培养的人类白细胞的动力学模式 通过用 111-铟（一种放射性元素）在离体细胞上进行标记来研究 同位素标记，并通过测量它们在体内的分布 伽马相机成像和血液样本伽马计数的方法。这 研究类型具有广泛的应用。最常见的用途是这些 放射性标记的白细胞运输研究用于脓肿定位 疑似感染病例未经其他非确诊者明确诊断 侵入性, 研究.在这些情况下，自体或同种异体粒细胞， 通过简单的静脉切开术或单采术收集，标有 50 muCi 每公斤患者体重 11 l-铟。为此目的进行了十四项研究 对临床中心患者进行脓肿定位 在过去 6 年里参加了各种协议。这 14 个中的两个 呈阳性并导致沙门氏菌病例得到适当治疗 HIV感染的血友病患者的骨髓炎和切除术 佐林格-埃利森综合征患者的憩室脓肿。在 许多负面研究表明，不必要的手术探查 避免了。 还对铟标记的白细胞运输研究进行了调查 作为诊断工具——检测患者的大血管炎症 患有高安氏动脉炎。合作进行的一项前瞻性研究 NIAID 和 NMD/CC 表明放射性标记的自体单核细胞 细胞运输无法准确预测患者是否有症状 伴有发烧和高沉降率的患者患有活动性动脉炎，因此 不能用于确定是否进行抗炎干预 应启动代理。 与 NCI 外科分部的合作试验调查了 放射性标记自体组织的诊断效用和预后应用 转移性患者的肿瘤浸润淋巴细胞（TIL）研究 黑色素瘤。 TIL 贩运研究揭示了转移性沉积物 临床上未检测到，并且 TIL 向肿瘤部位的运输强烈 与 TIL 细胞输注后的肿瘤消退相关 环磷酰胺。 最近，放射性标记的同种异体粒细胞运输研究 已被用于确定患者是否存在肺部浸润 儿童慢性肉芽肿病 (COD) 是由于感染或 肺部疤痕和肉芽肿形成。 COD 中的人口贩运研究 具有 HLA 同种免疫的患者已被用来预测是否 同种异体细胞在输血后将存活，因此是否 粒细胞输注的治疗试验是必要的。