ETIOLOGY OF ALLERGIC REACTIONS IN PLATELETPHERESIS DONORS

血小板去除术供者过敏反应的病因

• 批准号: 3939427
• 负责人: S F LEITMAN
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939427
• 关键词: allergens; basophils; blood donor; cell sorting; ethylenes; granulocyte; histamine; hypersensitivity; immunoglobulin E; immunologic skin test; plasmapheresis; plateletpheresis; radioimmunoassay

In February, 1984, the DTM converted from manual to automated platelet collection techniques, acquiring for this purpose four automated cell separation devices. During the next 3 years, 11 donors undergoing apheresis procedures on the Fenwal CS-3000 device experienced acute hypersensitivity reactions. Nine reactions occurred during plateletpheresis and 4 reactions occurred during granulocytapheresis procedures. The purpose of this study was to identify the allergen responsible for these reactions. Using a combination of skin tests, radioallergosorbent tests, and basophil histamine release assays, specific IgE- mediated sensitization to ethylene oxide, a gas used to sterilize the plastic disposable apheresis kits, was found in all plateletpheresis donors experiencing reactions, but in none of 100 non-reacting controls. Preliminary data also suggests that ethylene oxide is the agent responsible for hypersensitivity reactions during granulocytapheresis. It is estimated that as many as 1.0% of all repeat apheresis donors may become sensitized to ethylene oxide and experience allergic symptoms during donation. Although none of these reactions have been life- threatening, the DTM is continuing to test apheresis donors from sensitization to ethylene oxide, and to defer sensitized individuals from subsequent CS-3000 donations.

1984年2月，DTM由手动转为自动