LARGE-SCALE PRODUCTION & PURIFICATION OF COMPOUNDS WITH BIOLOGICAL ACTIVITY

生物技术股负责生产和生产 生物材料的提纯。该股承担着广泛的任务 生物生产任务，如大规模培养原核生物 细胞和真核细胞，各种化合物的生产 微生物和真核细胞，以及生物的纯化 化合物，尤指蛋白质。这些产品不在以下网站提供 临床和结构研究需要商业来源。这个 生物技术单位有能力处理各种生产问题 与广泛不同的主题关联，通过实现 生理和技术方法。高效的流程开发 生产是可能的，因为该单位拥有两个一体化的 要素：一个全面运营的多用途中试生产设施 以及进行研究和工艺开发的能力。 在过去的一年里，该部队表演了大约200种不同的， 大规模制剂：1)培养各种微生物，如 重组大肠杆菌、重组酿酒酵母、 重组毕赤酵母、百日咳杆菌突变株、 白喉棒状杆菌突变株和几种类型的 沙门氏菌和志贺氏菌。2)大量传播各种不同的 哺乳动物细胞，以及3)提纯克量的白喉毒素 交叉反应材料与重组铜绿假单胞菌 外毒素A。在研究和开发领域，该单位继续： 1)它致力于开发更有效的方法来解决 规模化蛋白质回收，实施扩张床实验 在离子交换器上捕获蛋白质的吸收技术和 2)开展研究工作，旨在了解 不同重组大肠杆菌菌株的葡萄糖代谢 确定为什么某些菌株更适合外源蛋白质 生产；3)探索锚地繁殖方法。 依赖哺乳动物的细胞，如细胞立方体和流态化床。

The Biotechnology Unit is responsible for the production and purification of biological material. The Unit undertakes a wide array of biological production tasks such as large-scale growth of prokaryotic cells and eucaryotic cells, production of various compounds from microorganixms and eucaryotic cells, and purification of biological compounds especially proteins. These products, not available from commercial sources, are needed for clinical and structural studies. The Biotechnology Unit is capable of handling various production problems associated with widely different topics by implementing both physiological and technical approaches. Efficient process development and production is possible since the Unit possesses two integrated elements: a fully operational multi-purpose pilot production facility and the capability of conducting research and process development. During the last year the unit performed approximately 200 different, large scale preparations: 1) Growing various microorganisms such as recombinant Escherichia coli, recombinant Saccharomyces cerevisiae, recombinant Pichia pastoria, mutant strains of Bortedella pertussis, mutant strains of Corynebacteria diphtheria and several types of Salmonella and Shigella. 2) Propagating large volumes of various mammalian cells, and 3) purifying gram quantities of diphtheria toxin CRM (Cross Reacting Material) and recombinant pseudomonas aeruginosa exotoxin A. In the research and development area the unit continues: 1) its effort on the development of more efficient methods for large scale protein recovery, performing experiments implementing expanded bed absorption techniques for capturing proteins on an ion exchangers and chelating resins; 2) conducting research work aimed at understanding the glucose metabolism of various recombinant E. coli strains, in an effort to determine why certain strains are more suitable for foreign protein production; and 3) exploring methods for propagation of anchorage dependent mammalian cells, such as cell cubes and fluidized bed.