LARGE-SCALE PRODUCTION & PURIFICATION OF COMPOUNDS WITH BIOLOGICAL ACTIVITY

生物技术股负责生产和 生物材料的纯化。该股开展了一系列广泛的工作， 生物生产任务，如原核生物的大规模生长， 细胞和真核细胞，产生各种化合物 微生物和真核细胞，以及生物纯化 化合物，尤其是蛋白质。这些产品，从 商业来源，用于临床和结构研究。 的 生物技术部门有能力处理各种生产问题 与广泛不同的主题相关联， 生理和技术方法。高效的工艺开发 生产是可能的，因为该单位拥有两个集成的 要素：一个全面运作的多用途试验生产设施 以及进行研究和工艺开发的能力。 在过去的一年里，该单位执行了大约200个不同的， 大规模制备：1）培养各种微生物， 重组大肠杆菌，重组酿酒酵母， 重组巴斯德毕赤酵母，百日咳博德特氏菌突变株， 白喉棒状杆菌的突变菌株和几种类型的 沙门氏菌和志贺氏菌。2)大量收集各种 哺乳动物细胞，和3）纯化克量的白喉毒素， CRM（交叉反应物质）和重组铜绿假单胞菌 外毒素A 在研究和发展领域，该股继续： 1)它努力发展更有效的方法， 规模蛋白质回收，进行实验，实施膨胀床 用于在离子交换剂上捕获蛋白质的吸收技术， 螯合树脂; 2）进行研究工作，旨在了解 各种重组E.大肠杆菌菌株， 以确定为什么某些菌株更适合外源蛋白 3）探索繁殖锚地的方法 依赖性哺乳动物细胞，如细胞立方体和流化床。

The Biotechnology Unit is responsible for the production and purification of biological material. The Unit undertakes a wide array of biological production tasks such as large-scale growth of prokaryotic cells and eucaryotic cells, production of various compounds from microorganixms and eucaryotic cells, and purification of biological compounds especially proteins. These products, not available from commercial sources, are needed for clinical and structural studies. The Biotechnology Unit is capable of handling various production problems associated with widely different topics by implementing both physiological and technical approaches. Efficient process development and production is possible since the Unit possesses two integrated elements: a fully operational multi-purpose pilot production facility and the capability of conducting research and process development. During the last year the unit performed approximately 200 different, large scale preparations: 1) Growing various microorganisms such as recombinant Escherichia coli, recombinant Saccharomyces cerevisiae, recombinant Pichia pastoria, mutant strains of Bortedella pertussis, mutant strains of Corynebacteria diphtheria and several types of Salmonella and Shigella. 2) Propagating large volumes of various mammalian cells, and 3) purifying gram quantities of diphtheria toxin CRM (Cross Reacting Material) and recombinant pseudomonas aeruginosa exotoxin A. In the research and development area the unit continues: 1) its effort on the development of more efficient methods for large scale protein recovery, performing experiments implementing expanded bed absorption techniques for capturing proteins on an ion exchangers and chelating resins; 2) conducting research work aimed at understanding the glucose metabolism of various recombinant E. coli strains, in an effort to determine why certain strains are more suitable for foreign protein production; and 3) exploring methods for propagation of anchorage dependent mammalian cells, such as cell cubes and fluidized bed.