LARGE-SCALE PRODUCTION & PURIFICATION OF COMPOUNDS WITH BIOLOGICAL ACTIVITY

大规模生产

• 批准号: 2572772
• 负责人: J SHILOACH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2572772
• 关键词: biological products; biotechnology; chemical registry /resource; diphtheria toxin; genetic promoter element; immunological substance; microorganism mass culture; microorganism resource /registry; mitogen activated protein kinase; protein purification; recombinant DNA; tissue /cell culture

The Biotechnology Unit is responsible for the production and purification of biological material. The Unit undertakes a wide array of biological production tasks such as large-scale growth of procaryotic cells and eucaryotic cells, production of various compounds from microorganisms and eucaryotic cells, and purification of biological compounds especially proteins. These products, not available from commercial sources, are needed for clinical and structural studies. The Biotechnology Unit is capable of handling various production problems associated with widely different topics by implementing both physiological and technical approaches. Efficient process development and production is possible since the Unit possesses two integrated elements: a fully operational multi- purpose pilot production facility and the capability of conducting research and process development. During the last year the Unit performed approximately 200 different large-scale preparations: (1) growing various microorganisms such as recombinant E. coli, recombinant yeast, Corynebacteria diphtheria, Vibrio cholera, and several types of Shigella; (2) propagating large volumes of various mammalian cells; and (3) purifying gram quantities of several proteins such as CRM (cross-reacting material) diphtheria toxin and bovine S antigen. The Unit concentrates on the development of more efficient methods for protein recovery. In this area research was conducted for the use of expanded bed technique for the recovery of both intracellular and extracellular proteins. A new method for propagating of anchorage dependent mammalian cells for the production of secreted proteins was also introduced.

生物技术股负责生产和 生物材料的纯化。 该股开展了一系列广泛的工作， 生物生产任务，如大规模增长， 原核细胞和真核细胞，生产各种 来自微生物和真核细胞的化合物和纯化 生物化合物，尤其是蛋白质。 这些产品，不 可从商业来源获得，用于临床和 结构研究。 生物技术部门能够处理各种生产 与广泛不同的主题相关联的问题， 生理和技术方法。 高效的工艺开发 生产是可能的，因为该单位拥有两个集成的 元素：一个全面运作的多用途试验生产 进行研究和加工的设施和能力 发展 在过去一年中，该股执行了大约200个不同的 大规模制备：（1）培养各种微生物， 重组大肠大肠杆菌，重组酵母，白喉棒状杆菌， 霍乱弧菌和几种志贺氏菌;（2）繁殖大 各种哺乳动物细胞的体积;和（3）纯化克量 几种蛋白质，如CRM（交叉反应物质）白喉 毒素和牛S抗原。 该股的工作重点是制定更有效的方法， 蛋白质回收 在这一领域进行了研究， 膨胀床技术用于回收细胞内和 胞外蛋白质 一种新的锚定传播方法 依赖于哺乳动物细胞的分泌蛋白的生产， 还介绍