LARGE-SCALE PROCESSING OF BIOLOGICAL MATERIALS

生物材料的大规模加工

• 批准号: 3917366
• 负责人: J SHILOACH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3917366
• 关键词: Escherichia coli; Mammalia; Pseudomonas aeruginosa; bacterial polysaccharides; bacterial toxins; biological products; biotechnology; enzyme structure; eukaryote; exotoxins; microorganism culture; microorganism mass culture; microorganism resource /registry; neoplastic cell; peptide hormone; pertussis; prokaryote; retinoblastoma; tissue /cell culture; tissue resource /registry

The Pilot Plan (Biotechnology) Unit combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it is conducting process development work associated with these preparations In order to be able to execute them efficiently. In addition, the unit is carrying on research work not necessarily associated with a current project, but work that has long-term implications for the unit's performance. During the last year, the unit carried out 150 different large- scale preparations, including microorganism growth in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Development work was done in the optimization of E. coli growth using the fed batch technique and oxygen-enriched air yielding 100 g/liter wet weight of biomass. A new method for the purification of Exotoxin A from pseudomonas aeruginosa was developed.

试验计划（生物技术）股结合了几个不同的 活动类型。 它负责大规模的 生产细菌、哺乳动物细胞和生物活性 各种来源的化合物。 与此同时， 开展与这些相关的流程开发工作 为了能够有效地执行它们。 在 此外，单位不一定在进行研究工作， 与当前项目相关，但具有长期性的工作 对单位业绩的影响。 去年，该单位开展了150项不同的大型- 规模制剂，包括微生物生长量， 10至300升，哺乳动物细胞生长高达50升体积， 加工各种生物材料。 在优化E.杆菌生长 使用进料分批技术和富氧空气产生100 g/升生物质湿重。 一种新的纯化方法 铜绿假单胞菌外毒素A的分离纯化。