LARGE-SCALE PROCESSING OF BIOLOGICAL MATERIALS

生物材料的大规模加工

• 批准号: 3875545
• 负责人: J SHILOACH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3875545
• 关键词: Escherichia coli; bacterial toxins; biological products; biotechnology; exotoxins; genetic recombination; mass tissue /cell culture; microorganism mass culture; microorganism resource /registry; tissue /cell culture; tissue resource /registry

The Pilot Plant (Biotechnology Unit) combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it conducts process development work associated with these preparations in order to be able to execute them efficiently. In addition, the unit carries on research work not necessarily associated with a current project, but work that has long-term implications for the unit's performance. During the last year, the unit carried out 123 different large-scale preparations, including microorganism growth in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Development work was done in the growth optimization of various recombinant E. coli. An example is growth and recovery process for obtaining gr quantities of purified non-toxic exotoxin A lacking glutamic acid at position 553.

中试工厂（生物技术单元）结合了几种不同类型的 活动它负责细菌的大规模生产， 哺乳动物细胞和各种来源的生物活性化合物。 与此活动并行的是， 这些准备工作，以便能够有效地执行它们。在 此外，该股进行的研究工作不一定与 一个当前的项目，但工作有长期的影响，该单位的 性能 在过去的一年里，该单位进行了123个不同的大规模 制剂，包括10至300体积的微生物生长 升，哺乳动物细胞生长高达50升的体积和处理 各种生物材料。 在各种重组体的生长优化方面进行了开发工作 E.杆菌一个例子是获得gr的增长和恢复过程 纯化的无毒外毒素A缺乏谷氨酸， 位置553。