LARGE-SCALE PROCESSING OF BIOLOGICAL MATERIAL

生物材料的大规模加工

• 批准号: 3854538
• 负责人: J SHILOACH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854538
• 关键词: Escherichia coli; Pseudomonas aeruginosa; bacterial toxins; biotechnology; chemical registry /resource; exotoxins; microorganism growth; microorganism mass culture; microorganism resource /registry; protein purification; tissue /cell culture; tissue resource /registry

The Pilot Plant (Biotechnology Unit) combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it conducts Process development work associated with these preparations in order to be able to execute them efficiently. In addition, the unit carries on research work not necessarily associated with a current project, but work that has long-term implications for the Unit's performance. During the last year, the Unit produced 185 different large-scale preparations, including microorganism grown in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Process development work was done on large-scale purification of two proteins needed for the first phase of clinical trials. One is a modified form of Pseudomonas aeruginosa exotoxin A from recombinant E. coli and the second is S antigen from bovine retinas. Research was conducted on developing an adaptive control strategy for maintaining the dissolved oxygen level in the aerobic growth of microorganisms.

中试工厂（生物技术单元）结合了几种不同类型的 活动 它负责细菌的大规模生产， 哺乳动物细胞和各种来源的生物活性化合物。 与此活动并行的是，它还开展相关的流程开发工作， 这些准备工作，以便能够有效地执行它们。 此外，该单位进行的研究工作不一定与 目前的项目，但工作有长期的影响， 单位的业绩。 在过去的一年里，该股生产了185个不同的大型 制备物，包括在10至300体积中生长的微生物 升，哺乳动物细胞生长高达50升的体积和处理 各种生物材料。 对两种化合物的大规模纯化进行了工艺开发工作， 第一阶段临床试验所需的蛋白质。 一种是改良的 从重组E.杆菌和 其次是牛视网膜S抗原。 研究开发了一种自适应控制策略， 维持好氧生长中的溶解氧水平， 微生物的