Do Archaea dominate nitrification in acid soils?

古细菌在酸性土壤中主导硝化作用吗？

• 批准号: NE/F021909/1
• 负责人: Graeme Nicol
• 金额: $ 36.05万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FF021909%2F1
• 关键词: Do; Archaea; dominate; nitrification; acid

Which organisms are responsible for nitrogen cycling in acid soils? Nitrification is essential to the cycling of nitrogen throughout the environment. It involves the conversion of ammonia to nitrate by two groups of microorganisms. Ammonia oxidisers convert ammonia to nitrite, which is subsequently converted to nitrite by nitrite oxidisers. Although an essential process, nitrification can also have deleterious consequences. Ammonium can be retained in soil, but after conversion to nitrate, it can be readily leached resulting in the pollution of groundwaters that may be used to supply drinking water. In addition, ammonia oxidation is accompanied by the production of nitrous oxide, a potent greenhouse gas which is also involved in destroying stratospheric ozone in the atmosphere. Approximately 30% of the world's soil can be considered acid (with a pH less than 5.5) and represent a wide range of natural and managed habitats including forestry, agriculture and grasslands. They are therefore of great environmental and economic importance. In contrast to other soils (such as managed arable land), the processes and organisms responsible for nitrification in acid soils are not fully understood, as typical ammonia oxidisers that were thought to be primarily responsible for ammonia oxidation are unable to grow in laboratory media at acidic pH. Until recently it was believed that the most important soil ammonia oxidisers were bacteria. However, this view has changed with the recent discovery of another group of abundant group of organisms that possess the ability to oxidise ammonia, the Crenarchaea. These organisms belong to another domain of microbial life, the archaea. Although they resemble bacteria in many ways, they are evolutionary distinct. These organisms were once thought to be restricted to hyperthermophilic environments such as deep-sea vents or terrestrial thermal springs. However, with the use of molecular techniques, these organisms have been demonstrated to be ubiquitous in the environment. We have recently obtained evidence that in one experimental field site, ammonia oxidising Crenarchaea show greatest activity in acidic soils, in direct contrast to ammonia oxidising bacteria which show greatest activity in neutral soils. This therefore indicates that there are fundamental ecological and physiological differences between these two groups. This research programme aims to determine whether soil crenarchaea, rather than bacteria, are primarily responsible for ammonia oxidation in acid soils from a range of different habitats. Their abundance will be measured in acidic soils and neighbouring neutral pH soils from four sites from within the UK, and their relative abundance will also be determined in forest and pasture soils from three other countries (Finland, Canada and Australia). The findings will benefit those in agriculture, forestry and industry by increasing our knowledge of the organisms responsible for nitrogen cycling in natural and managed acidic soil systems. The findings will also be important to researchers trying to understand the links between biodiversity and ecosystem function, including the mechanisms and organisms potentially responsible for the emission of nitrous oxide from soil.

酸性土壤中哪些生物负责氮循环？硝化作用对氮在整个环境中的循环至关重要。它涉及两组微生物将氨转化为硝酸盐。氨氧化剂将氨转化为亚硝酸盐，亚硝酸盐随后被亚硝酸盐氧化剂转化为亚硝酸盐。虽然硝化是一个重要的过程，但它也可能产生有害的后果。铵可以保留在土壤中，但在转化为硝酸盐后，它很容易浸出，导致可能用于供应饮用水的地下水污染。此外，氨氧化还伴随着一氧化二氮的产生，这是一种强效温室气体，也参与破坏大气中的平流层臭氧。世界上大约30%的土壤被认为是酸性的（pH值低于5.5），代表了广泛的自然和管理栖息地，包括林业，农业和草原。因此，它们具有重大的环境和经济意义。与其他土壤（如管理耕地）相比，酸性土壤中硝化作用的过程和生物体尚未完全了解，因为被认为主要负责氨氧化的典型氨氧化剂无法在酸性pH的实验室介质中生长。然而，随着最近发现另一组具有氧化氨能力的丰富生物群，Crenarchaea，这种观点已经改变。这些生物属于微生物生命的另一个领域，即古细菌。虽然它们在许多方面与细菌相似，但它们在进化上是不同的。这些生物曾被认为仅限于深海喷口或陆地温泉等极端嗜热环境。然而，随着分子技术的使用，这些生物体已被证明是无处不在的环境中。我们最近获得的证据表明，在一个实验现场，氨氧化Crenarchea显示最大的活动在酸性土壤中，在直接对比氨氧化细菌，在中性土壤中显示最大的活动。因此，这表明这两个群体之间存在根本的生态和生理差异。这项研究计划的目的是确定是否土壤crenarchaea，而不是细菌，主要负责从一系列不同的栖息地酸性土壤中的氨氧化。它们的丰度将在英国境内四个地点的酸性土壤和邻近的中性pH土壤中进行测量，它们的相对丰度也将在其他三个国家（芬兰、加拿大和澳大利亚）的森林和牧场土壤中进行测定。这些发现将使农业，林业和工业受益，增加我们对自然和管理的酸性土壤系统中负责氮循环的生物体的了解。这些发现对于试图了解生物多样性与生态系统功能之间联系的研究人员也很重要，包括可能导致土壤中一氧化二氮排放的机制和生物。

项目成果

Ammonia-oxidising archaea living at low pH: Insights from comparative genomics.

• DOI: 10.1111/1462-2920.13971
• 发表时间: 2017-12
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Herbold CW;Lehtovirta-Morley LE;Jung MY;Jehmlich N;Hausmann B;Han P;Loy A;Pester M;Sayavedra-Soto LA;Rhee SK;Prosser JI;Nicol GW;Wagner M;Gubry-Rangin C
• 通讯作者: Gubry-Rangin C

Characterisation of terrestrial acidophilic archaeal ammonia oxidisers and their inhibition and stimulation by organic compounds.

陆地嗜酸古菌氨氧化剂的表征及其有机化合物的抑制和刺激

• DOI: 10.1111/1574-6941.12353
• 发表时间: 2014-09
• 期刊: FEMS microbiology ecology
• 影响因子: 4.2
• 作者: Lehtovirta-Morley LE;Ge C;Ross J;Yao H;Nicol GW;Prosser JI
• 通讯作者: Prosser JI

Identifying Potential Mechanisms Enabling Acidophily in the Ammonia-Oxidizing Archaeon "Candidatus Nitrosotalea devanaterra".

• DOI: 10.1128/aem.04031-15
• 发表时间: 2016-05
• 期刊: Applied and environmental microbiology
• 影响因子: 4.4
• 作者: Lehtovirta-Morley LE;Sayavedra-Soto LA;Gallois N;Schouten S;Stein LY;Prosser JI;Nicol GW
• 通讯作者: Nicol GW

Temperature responses of soil ammonia-oxidising archaea depend on pH

• DOI: 10.1016/j.soilbio.2016.12.007
• 发表时间: 2017-03-01
• 期刊: SOIL BIOLOGY & BIOCHEMISTRY
• 影响因子: 9.7
• 作者: Gubry-Rangin, Cecile;Novotnik, Breda;Prosser, James I.
• 通讯作者: Prosser, James I.

Isolation of 'Candidatus Nitrosocosmicus franklandus', a novel ureolytic soil archaeal ammonia oxidiser with tolerance to high ammonia concentration.

• DOI: 10.1093/femsec/fiw057
• 发表时间: 2016-05
• 期刊: FEMS microbiology ecology
• 影响因子: 4.2
• 作者: Lehtovirta-Morley LE;Ross J;Hink L;Weber EB;Gubry-Rangin C;Thion C;Prosser JI;Nicol GW
• 通讯作者: Nicol GW