DSRNAS OF CRYPTOSPORIDIUM PARVUM

小隐孢子虫的 DSRNA

• 批准号: 6170902
• 负责人: Steve J Upton
• 金额: $ 16.53万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-15 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6170902
• 关键词: Cryptosporidium; antisense nucleic acid; chemical structure function; developmental genetics; double stranded RNA; gene expression; immunoelectron microscopy; life cycle; nucleic acid structure; protein kinase

DESCRIPTION (Adapted from the Applicant's Abstract): Cryptosporidium parvum is a gastrointestinal parasite capable of causing moderate to severe diarrheal disease in both humans and animals. The parasite is ubiquitous and is known to infect at least 79 different species of animals originating from more than 50 countries. Recent studies have demonstrated the presence of two high copy number, linear, double- stranded RNAs (dsRNAs) within sporozoites of 6 tested isolates of C. parvum, but not in 6 other species of the genus. Each of the DsRNAs has been purified and a library of cDNA clones representing both segments synthesized. Analysis of sequences has revealed the large segment (1786 nt) may encode and RNA-dependent RNA polymerase (RDRP), and the small segment (1374 nt) a putative protein kinase with limited similarity to mitogen-activated JNK protein kinase. No capsid protein has yet been found that co-purifies with the segments. The dsRNAs are extrachromosomal genetic element as they have no copies in the C. parvum genome, and the RDRP has an unusually high optimum temperature in vitro (50 degrees C). Investigators at Kansas State University propose to study the structure and function of the dsRNAs in C. parvum, as well as the putative proteins encoded by the dsRNA genes. Should these viral- like RNAs be found to encode proteins that modulate the parasite life- cycle, or be shown to affect the ability of the parasite to survive or induce pathogenicity, then they may also be found to represent useful therapeutic targets.

描述（改编自申请人的摘要）：隐孢子虫 小寄生虫是一种胃肠道寄生虫， 人类和动物的一种严重的传染病。 述寄生虫是 普遍存在，已知感染至少79种不同的 来自50多个国家的动物。 最近的研究 证明存在两个高拷贝数，线性，双- 6株C. parvum，但没有在该属的其他6种。 每一个DsRNA都有 纯化，并构建了代表这两个片段的cDNA克隆文库 合成了 序列分析揭示了大片段（1786 nt）可能编码依赖于RNA的RNA聚合酶（RDRP），而小的 片段（1374 nt）一个假定的蛋白激酶，与 丝裂原活化JNK蛋白激酶。 目前还没有衣壳蛋白 发现它与片段共同净化。 dsRNA是 染色体外遗传元件，因为它们在C. parvum RDRP在体外具有异常高的最适温度 (50摄氏度）。 堪萨斯州立大学的调查人员建议， 研究C. parvum，以及 由dsRNA基因编码的推定蛋白质。 如果这些病毒- 类似的RNA被发现编码调节寄生虫生命的蛋白质- 周期，或被证明会影响寄生虫的生存能力， 诱导致病性，那么它们也可能被发现代表有用 治疗目标