Development of in-situ sensors for direct quantification of metal speciation and bio-availability

开发用于直接量化金属形态和生物利用度的原位传感器

• 批准号: NE/H002480/1
• 负责人: Hao Zhang
• 金额: $ 21.93万
• 依托单位: Lancaster University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FH002480%2F1
• 关键词: Development; situ; sensors; direct; quantification

Metals in the environment can be essential to life forms and so are regarded as micronutrients, but when excessive amounts are present they may be toxic. Current measurement and assessment of the metal that is available to biota is essential for optimising agriculture and aquaculture and for assessing ecological and human health. The uptake of metals by organisms is complicated and depends on the dynamics of exchange of the various chemical forms of the metal. For a measurement tool to assess successfully the bioavailable forms of the metal it must mimic the essential characteristics of the biota. The new technique of diffusive gradients in thin-films (DGT) emulates some aspects of the dynamics, but it lacks some essential features of the organism. This work will introduce controlled biological features into DGT to provide two new tools that can be used in situ to assess bioavailable metals. The biological binding DGT (BBDGT) will incorporate cell cultures to enable precise mimicry of uptake from solution. The biological mobilising DGT (BMDGT) will contain microbial components that can enable the measurement of metals capable of release from colloids and particles.

环境中的金属可能是生命形式所必需的，因此被视为微量营养素，但当过量时，它们可能是有毒的。目前测量和评估生物群可获得的金属对于优化农业和水产养殖以及评估生态和人类健康至关重要。生物体对金属的吸收是复杂的，取决于金属的各种化学形式的交换动力学。为了使测量工具能够成功地评估金属的生物可利用形式，它必须模拟生物群的基本特征。新技术的扩散梯度薄膜（DGT）模拟动力学的某些方面，但它缺乏一些基本特征的有机体。这项工作将引入可控的生物特征到DGT提供两个新的工具，可用于原位评估生物可利用的金属。生物结合DGT（BBDGT）将掺入细胞培养物，以精确模拟从溶液中摄取。生物动员DGT（BMDGT）将包含微生物成分，可以测量能够从胶体和颗粒中释放的金属。

项目成果

Immobilization of Shewanella oneidensis MR-1 in diffusive gradients in thin films for determining metal bioavailability.

• DOI: 10.1016/j.chemosphere.2015.06.018
• 发表时间: 2013-09
• 期刊: Chemosphere
• 影响因子: 8.8
• 作者: P. Baker;Christer Högstrand;J. Lead;R. Pickup;Hao Zhang