GLOBAL CONTROL OF DIFFERENTIATION IN CAULOBACTER

描述：新月芽孢杆菌的细胞分裂产生两个 不同的细胞类型：活动的丛生细胞和无柄的柄细胞。仅限 柄细胞能够进行DNA复制和细胞分裂。从长远来看 这个项目的目标是了解细胞分裂和细胞如何 铜绿假单胞菌的分化受到调控和协调。硫杆菌属 特别适合这些研究，因为它经历了一个相对 简单的发育程序，具有明显的形态，因为细胞 种群可以很容易地同步。第一个目标是定义 细胞周期与细胞分裂启动的发育调控 了解控制FtsZ的因素。作为这一目标的一部分，Dr。 布伦计划探索FtsZ特定细胞类型的蛋白分解机制， 确定FtsZ定位的发育和时间控制，以及 识别与FtsZ相互作用的蛋白质。第二个目标是 描述调控序列和负责反式作用的因素 调节ftsQA并确定ftsQA的转录如何与 DNA复制。最终的目标是确定依赖于sigma-54的 将极点发育与细胞分裂联系起来并定义 StpA的功能，这似乎是正确本地化所必需的 史迪克和霍法斯特。

DESCRIPTION: Cell division in the bacterium Caulobacter crescentus produces two different cell types: a motile swarmer cell and a sessile stalked cell. Only the stalked cell is capable of DNA replication and cell division. The long term goal of this project is to understand how cell division and cell differentiation are regulated and coordinated in Caulobacter. Caulobacter is particularly well suited for these studies because it undergoes a relatively simple developmental program with distnct morphologies, and because cell populations can be synchronized easily. The first objective is to define the cell cycle and developmental controls of cell division initiation through an understanding of the factors that control FtsZ. As part of this objective, Dr. Brun plans to explore the mechanism of cell type-specific proteolysis of FtsZ, determine the developmental and temporal control of FtsZ localization, and identify proteins that interact with FtsZ. The second objective is to characterize the regulatory sequences and trans-acting factors responsible for regulation of ftsQA and determine how the transcription of ftsQA is coupled to DNA replication. The final objective is to identify the sigma-54-dependent genes that are required to link pole development to cell division and to define the function of stpA, which appears to be required for proper localization of the stalk and holdfast.