Mechanism of Caulobacter adhesion

柄杆菌粘附机制

基本信息

  • 批准号:
    7212666
  • 负责人:
  • 金额:
    $ 31.2万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-02-01 至 2011-01-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The adhesion of bacteria to surfaces plays an important role in disease, providing the critical first step in the biofouling of a surface and in biofilm formation. The general goal of this research is to reach a detailed understanding of the mechanisms of bacterial adhesion, from the biophysics of adhesion to the coordination of the biosynthesis of cell surface structures that participate in this process. This project takes advantage of the bacterium Caulobacter crescentus, in which adhesive structures are synthesized in an ordered fashion at the same pole of the cell, making the study of adhesion more amenable than in most bacteria. Initial stages of adhesion involve flagellar motility and pili, and adhesion is cemented by synthesis of a polysaccharide holdfast. Pathogenic bacteria also use these adhesive structures, but their mechanism of action in adhesion is poorly understood. In addition, the adhesive force of individual Caulobacter cells is the strongest ever measured for a biological adhesive. This project has three major aims. The first aim will use highly synchronized cultures coupled to atomic force microscopy, fluorescence microscopy, and biophysical modeling to develop a detailed understanding of the various stages of adhesion. In particular, this aim will investigate a newly discovered surface contact-dependent trigger of adhesive polysaccharide export; this mechanism may also be used by pathogens. The second aim is to determine the function of holdfast polysaccharide synthesis and attachment proteins. Experiments are described to determine the biochemical function of these proteins and their contribution to adhesion. The third aim is to elucidate the mechanisms that control the timing and polar localization of holdfast synthesis. The effect of constitutively expressing holdfast synthesis proteins during the cell cycle on the timing of holdfast synthesis and adhesion will be determined. The localization of holdfast synthesis and attachment proteins and their interdependence for localization will be studied, and factors required for their localization will be identified. Bacterial adhesion, polysaccharide biosynthesis, and subcellular localization of proteins and virulence factors are essential components of bacterial pathogenesis. Insight gained from the study of this simple model system will be applicable to more complex bacterial pathogens and will enhance our ability to inhibit them.
描述(由申请人提供):细菌对表面的粘附在疾病中起着重要作用,为表面的生物污垢和生物膜形成提供了关键的第一步。本研究的总体目标是详细了解细菌粘附的机制,从粘附的生物物理学到参与该过程的细胞表面结构的生物合成的协调。该项目利用了细菌Caulobacter crescentus,其中粘附结构在细胞的同一极以有序的方式合成,使得粘附的研究比大多数细菌更容易进行。粘附的初始阶段涉及鞭毛运动和皮利,并且粘附通过多糖固着物的合成而得以巩固。致病细菌也使用这些粘附结构,但它们在粘附中的作用机制知之甚少。此外,单个柄杆菌属细胞的粘附力是生物粘合剂有史以来测量的最强的。该项目有三个主要目标。第一个目标将使用高度同步的文化耦合到原子力显微镜,荧光显微镜,和生物物理建模,以制定一个详细的了解粘附的各个阶段。特别是,这一目标将调查一个新发现的表面接触依赖性触发的粘附多糖出口,这种机制也可能被病原体使用。第二个目的是确定固着多糖合成和附着蛋白的功能。实验描述,以确定这些蛋白质的生化功能和它们的贡献粘附。第三个目的是阐明控制holdfast合成的时间和极性定位的机制。将确定在细胞周期中组成型表达固着合成蛋白对固着合成和粘附时间的影响。将研究固着合成和附着蛋白的定位及其相互依赖性,并确定其定位所需的因素。细菌粘附、多糖生物合成、蛋白质和毒力因子的亚细胞定位是细菌致病的基本组成部分。从这个简单的模型系统的研究中获得的见解将适用于更复杂的细菌病原体,并将提高我们抑制它们的能力。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

YVES V BRUN其他文献

YVES V BRUN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('YVES V BRUN', 18)}}的其他基金

Bacterial Subcellular Organization and its Impact on Growth, Development, Aging, and Surface Adhesion
细菌亚细胞组织及其对生长、发育、衰老和表面粘附的影响
  • 批准号:
    9276966
  • 财政年份:
    2017
  • 资助金额:
    $ 31.2万
  • 项目类别:
Dynamics of bacterial peptidoglycan synthesis
细菌肽聚糖合成动力学
  • 批准号:
    9197654
  • 财政年份:
    2015
  • 资助金额:
    $ 31.2万
  • 项目类别:
Dynamics of bacterial peptidoglycan synthesis
细菌肽聚糖合成动力学
  • 批准号:
    8809735
  • 财政年份:
    2015
  • 资助金额:
    $ 31.2万
  • 项目类别:
2014 Bacterial Cell Surfaces Gordon Research Conference
2014年细菌细胞表面戈登研究会议
  • 批准号:
    8785778
  • 财政年份:
    2014
  • 资助金额:
    $ 31.2万
  • 项目类别:
Synthesis and properties of a bacterial bioadhesive
细菌生物粘附剂的合成及性能
  • 批准号:
    8344340
  • 财政年份:
    2012
  • 资助金额:
    $ 31.2万
  • 项目类别:
Synthesis and properties of a bacterial bioadhesive
细菌生物粘附剂的合成及性能
  • 批准号:
    8518406
  • 财政年份:
    2012
  • 资助金额:
    $ 31.2万
  • 项目类别:
Synthesis and properties of a bacterial bioadhesive
细菌生物粘附剂的合成及性能
  • 批准号:
    8656372
  • 财政年份:
    2012
  • 资助金额:
    $ 31.2万
  • 项目类别:
Mechanism of Caulobacter adhesion
柄杆菌粘附机制
  • 批准号:
    8123689
  • 财政年份:
    2010
  • 资助金额:
    $ 31.2万
  • 项目类别:
Mechanism of Caulobacter adhesion
柄杆菌粘附机制
  • 批准号:
    7765561
  • 财政年份:
    2007
  • 资助金额:
    $ 31.2万
  • 项目类别:
Mechanism of Caulobacter adhesion
柄杆菌粘附机制
  • 批准号:
    7340743
  • 财政年份:
    2007
  • 资助金额:
    $ 31.2万
  • 项目类别:

相似海外基金

I-Corps: Translation Potential of Peptidic Ensembles as Novel Bio-adhesives
I-Corps:肽整体作为新型生物粘合剂的转化潜力
  • 批准号:
    2409620
  • 财政年份:
    2024
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Standard Grant
Architectural design of active adhesives
活性粘合剂的结构设计
  • 批准号:
    2403716
  • 财政年份:
    2024
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Standard Grant
Design of non-swellable adhesives for brain surgery using cyclodextrin inclusion polymer
使用环糊精包合物聚合物脑外科不可溶胀粘合剂的设计
  • 批准号:
    23H01718
  • 财政年份:
    2023
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Meta-material adhesives for improved performance and functionalisation of bondlines
超材料粘合剂可提高粘合层的性能和功能化
  • 批准号:
    EP/W019450/1
  • 财政年份:
    2023
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Fellowship
Light-propelled dental adhesives with enhanced bonding capability
具有增强粘合能力的光驱动牙科粘合剂
  • 批准号:
    10741660
  • 财政年份:
    2023
  • 资助金额:
    $ 31.2万
  • 项目类别:
DMREF: Accelerating the Design of Adhesives with Nanoscale Control of Thermomechanical Properties
DMREF:通过热机械性能的纳米级控制加速粘合剂的设计
  • 批准号:
    2323317
  • 财政年份:
    2023
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Continuing Grant
Mag-Cure: A novel method for magnetically induced bonding and de-bonding of thermoset adhesives in the Automotive Industry
Mag-Cure:汽车行业中热固性粘合剂磁感应粘合和脱粘的新方法
  • 批准号:
    10062336
  • 财政年份:
    2023
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Collaborative R&D
Biodegradable, Biocompatible Pressure Sensitive Adhesives
可生物降解、生物相容性压敏粘合剂
  • 批准号:
    10677869
  • 财政年份:
    2022
  • 资助金额:
    $ 31.2万
  • 项目类别:
Poly(glycerol carbonate) pressure sensitive adhesives for the in vivo closure of alveolar pleural fistulae
用于体内闭合肺泡胸膜瘘的聚(甘油碳酸酯)压敏粘合剂
  • 批准号:
    10746743
  • 财政年份:
    2022
  • 资助金额:
    $ 31.2万
  • 项目类别:
Enhanced bio-production of difficult to make peptide ingredients for specialty adhesives and personal care
增强用于特种粘合剂和个人护理品的难以制造的肽成分的生物生产
  • 批准号:
    10021363
  • 财政年份:
    2022
  • 资助金额:
    $ 31.2万
  • 项目类别:
    Investment Accelerator
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了