GLOBAL CONTROL OF DIFFERENTIATION IN CAULOBACTER

描述： 新月柄杆菌的细胞分裂产生两种 不同的细胞类型：运动的群细胞和无柄的细胞。仅 有柄细胞能够进行DNA复制和细胞分裂。长期来看 该项目的目标是了解细胞分裂和细胞如何 柄杆菌中的分化受到调节和协调。茎杆菌是 特别适合这些研究，因为它经历了相对 具有不同形态的简单发育程序，并且因为细胞 群体可以轻松同步。第一个目标是定义 细胞周期和细胞分裂起始的发育控制 了解控制 FtsZ 的因素。作为这一目标的一部分，博士。 Brun计划探索FtsZ的细胞类型特异性蛋白水解机制， 确定 FtsZ 定位的发育和时间控制，以及 识别与 FtsZ 相互作用的蛋白质。第二个目标是 表征调节序列和反式作用因子 ftsQA 的调节并确定 ftsQA 的转录如何与 DNA复制。最终目标是确定 sigma-54 依赖性 将极发育与细胞分裂联系起来并定义 stpA 的功能，这似乎是正确定位 茎和固定器。

DESCRIPTION: Cell division in the bacterium Caulobacter crescentus produces two different cell types: a motile swarmer cell and a sessile stalked cell. Only the stalked cell is capable of DNA replication and cell division. The long term goal of this project is to understand how cell division and cell differentiation are regulated and coordinated in Caulobacter. Caulobacter is particularly well suited for these studies because it undergoes a relatively simple developmental program with distnct morphologies, and because cell populations can be synchronized easily. The first objective is to define the cell cycle and developmental controls of cell division initiation through an understanding of the factors that control FtsZ. As part of this objective, Dr. Brun plans to explore the mechanism of cell type-specific proteolysis of FtsZ, determine the developmental and temporal control of FtsZ localization, and identify proteins that interact with FtsZ. The second objective is to characterize the regulatory sequences and trans-acting factors responsible for regulation of ftsQA and determine how the transcription of ftsQA is coupled to DNA replication. The final objective is to identify the sigma-54-dependent genes that are required to link pole development to cell division and to define the function of stpA, which appears to be required for proper localization of the stalk and holdfast.