NIDDK BIOTECHNOLOGY CENTER

描述(应用程序摘要) 使用微阵列技术进行转录图谱分析已经 彻底改变了表征基因表达模式的方法 在生理和病理条件下的细胞内。这样做的目的是 NIDDK生物技术中心正在打造全面的集成微阵列 将允许N1DDK研究人员创建和分析定制和 商业表达阵列作为获得对疾病的新见解的工具 发病机理和机制，以及允许表征 疾病相关基因的功能和调控途径。来自这些的结果 研究最终将应用于诊断、药物筛选和治疗 选择不同的人类疾病状态。我们利用了一种 由NIDDK资助的哈佛社区和 贝丝以色列女执事医学的研究、教学和临床优势 儿童医院乔斯林糖尿病中心(BIDMC)，Dana Farber 癌症研究所(DFCI)和其他哈佛大学机构创建一个 多机构NIDDK生物技术中心。这项拨款申请的重点是 尤其是糖尿病、肥胖症和肾脏病的强项 我们院校的课程反映在10个评选项目中 正在进行的和3个可行性项目。中心的一个重要组成部分，在 除了技术核心，还包含了高度交互的 生物信息学核心。该中心的优势之一将是独特的 有能力提供网上注释、编目设施和 为所有NIDDK调查人员提供最先进的生物信息学分析。这将是 使所有项目的研究人员能够最大限度地利用表情数据 集合，以确定已知基因之间的功能依赖关系并表达 序列标签(EST)，并指导对这些推定的进一步生物学验证 依赖关系。NIDDK生物技术中心将被组织成几个 核心：技术核心、生物信息学核心和教育核心 这将由行政核心进行监督和协调。NIDDK 生物技术中心将结合Affymetrix的互补技术 寡核苷酸微阵列技术、定制微阵列和实时 聚合酶链式反应和生物信息学，并将这些专业知识集中在糖尿病问题上， 肥胖和肾脏疾病。从这些调查中获得的知识应该 深入了解疾病的调控途径和机制 发病机制涉及糖尿病、肥胖和肾脏疾病。最后， 中心将提供新颖的教育项目以吸引其他NIDDK 研究人员使用微阵列技术并传播通过 调查。

DESCRIPTION (Abstract of the application) The use of microarray technologies for transcriptional profiling has revolutionized the approach for characterizing the pattern of gene expression in cells under physiological and pathological conditions. The goal of this NIDDK Biotechnology Center is to build a comprehensive integrated microarray facility that will allow N1DDK researchers to create and analyze customized and commercial expression arrays as tools to gain new insights into disease pathogenesis and mechanisms, as well as permitting the characterization of the functional and regulatory pathways of disease related genes. Results from these studies will ultimately be applied for diagnosis, drug screening and treatment selection for diverse human disease states. We capitalize on the existence of a unique group of NIDDK funded investigators in the Harvard community and on the research, teaching and clinical strengths of the Beth Israel Deaconess Medical Center (BIDMC), Joslin Diabetes Center (JDC), Children's Hospital, Dana Farber Cancer Institute(DFCI) and other Harvard Institutions to create a multi-institutional NIDDK Biotechnology Center. This grant application focuses particularly on the strength of the combined diabetes, obesity, and nephrology programs at our institutions which are reflected in the selection of the 10 ongoing and 3 feasibility projects. A critical component of the Center, in addition to the Technology Core, is the inclusion of a highly interactive Bioinformatics Core. One of the strengths of the Center will be the unique capacity to provide web-accessible annotation, cataloging facilities and state-of-the-art bioinformatics analyses to all NIDDK investigators. This will enable researchers from all Projects to maximally utilize the expression data sets to determine functional dependencies among the known genes and Expressed Sequence Tags (ESTs) and direct further biological validation of these putative dependencies. The NIDDK Biotechnology Center will be organized into several Cores: the Technology Core, the Bioinformatics Core, and the Education Core which will be supervised and coordinated by the Administrative Core. The NIDDK Biotechnology Center will combine the complementary technologies of Affymetrix oligonucleotide microarray technology, custom made microarrays, and real time PCR with bioinformatics, and focus this expertise on problems in diabetes, obesity, and kidney disease. Knowledge gained from these investigations should provide insights into regulatory pathways and mechanisms of disease pathogenesis involved in diabetes, obesity and kidney disease. Finally, the Center will provide novel educational programs to attract other NIDDK researchers to microarray technologies and to disseminate knowledge gained by the investigations.