DETERMINATION OF EMBRYONIC POLARITY
DETERMINATION OF EMBRYONIC POLARITY
批准号:
6180987
负责人:
VIRGINIA E. PAPAIOANNOU
金额:
$24.68万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-08-01 至 2001-07-31
关键词:
cell cell interaction cell migration cellular polarity chick embryo cytogenetics early embryonic stage gene expression genetic markers genetic regulation histogenesis immunoaffinity chromatography immunofluorescence technique in situ hybridization nucleic acid probes orientation polymerase chain reaction quail video microscopy western blottings
中文摘要
在胚胎发育的早期阶段,形成
英文摘要
During the early stages on embryonic development, the formation of the
embryonic axis is initiated at one pole of the embryo. Most of what is
known about the molecular mechanisms underlying this process is based
on amphibian embryos, where maternally-derived determinants control this
polarity. In amniotes (birds and mammals) this is unlikely because
embryos at much later stages can initiate axis formation at any
position. Here, the mechanisms that determine embryonic polarity in the
early stages of development will be studied using the chick embryo as
a model, because of their ease of manipulation and culture.
First, the role of the hypoblast (which had been suggested to be
responsible for polarity determination) will be tested directly. Then,
the region homologous to the amphibian "Nieuwkoop center" (which has
been shown in frogs to determine the position of future "organizer" and
axial cells) will be located. Finally, a number of genes already found
to be expressed in a way that predicts the future polarity of the embryo
will be placed in hierarchial order, taking advantage of the fact that
portions of the embryo will initiate axis formation when isolated from
the rest of the embryo.
The results of this project will be of value in suggesting how the
molecular cascades that have been implicated in the early development
of lower vertebrates (fish, amphibians) can be applied to higher
vertebrates (birds and mammals). Potentially, knowledge of these
cascades could lead to the development of new molecular diagnostic tools
for some of the many serious congenital disorders that affect the early
development of the embryonic axis, and may help determine whether
monozygotic and conjoined twinning are likely to have a molecular basis.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1006/jtbi.2000.2229
发表时间:
2001
期刊:
Journal of theoretical biology.
影响因子:
--
作者:
[Page,KM, Maini,PK, Monk,NA, Stern,CD]
通讯作者:
Stern,CD
Role of T-box genes in mouse development
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批准号:8051039
-
项目类别:
-
资助金额:$1.02万
-
财政年份:2010
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box genes in mouse development
-
批准号:7863895
-
项目类别:
-
资助金额:$1.02万
-
财政年份:2009
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
T-box gene effects on left-right body axis determination
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批准号:7895071
-
项目类别:
-
资助金额:$37.2万
-
财政年份:2009
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Islet growth in NOD mice tolerant to autoimmune diabetes
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批准号:6916401
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项目类别:
-
资助金额:$20.13万
-
财政年份:2004
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Islet growth in NOD mice tolerant to autoimmune diabetes
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批准号:6827263
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项目类别:
-
资助金额:$20.13万
-
财政年份:2004
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
ROLE OF TBX6 IN MESODERM PATTERING AND SOMITE FORMATION
-
批准号:6495218
-
项目类别:
-
资助金额:$3.82万
-
财政年份:2000
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
ROLE OF TBX6 IN MESODERM PATTERING AND SOMITE FORMATION
-
批准号:6520150
-
项目类别:
-
资助金额:$24.19万
-
财政年份:2000
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
ROLE OF TBX6 IN MESODERM PATTERING AND SOMITE FORMATION
-
批准号:6604714
-
项目类别:
-
资助金额:$23.51万
-
财政年份:2000
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
ROLE OF TBX6 IN MESODERM PATTERING AND SOMITE FORMATION
-
批准号:6195235
-
项目类别:
-
资助金额:$25.39万
-
财政年份:2000
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
ROLE OF TBX6 IN MESODERM PATTERING AND SOMITE FORMATION
-
批准号:6387067
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项目类别:
-
资助金额:$23.35万
-
财政年份:2000
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
EXPRESSION AND ROLE OF T-BOX GENES IN MOUSE DEVELOPMENT
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批准号:2872837
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项目类别:
-
资助金额:$24.28万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box Genes in Mouse Development
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批准号:6734732
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项目类别:
-
资助金额:$32.74万
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财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box genes in mouse development
-
批准号:8615919
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项目类别:
-
资助金额:$41.69万
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财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box genes in mouse development
-
批准号:8786825
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项目类别:
-
资助金额:$42.98万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box Genes in Mouse Development
-
批准号:6614344
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项目类别:
-
资助金额:$1.06万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box genes in mouse development
-
批准号:7354084
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项目类别:
-
资助金额:$39.01万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box Genes in Mouse Development
-
批准号:6620942
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项目类别:
-
资助金额:$32.66万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
Role of T-box Genes in Mouse Development
-
批准号:7002184
-
项目类别:
-
资助金额:$32.13万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
EXPRESSION AND ROLE OF T-BOX GENES IN MOUSE DEVELOPMENT
-
批准号:2332287
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项目类别:
-
资助金额:$22.45万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
EXPRESSION AND ROLE OF T-BOX GENES IN MOUSE DEVELOPMENT
-
批准号:2206459
-
项目类别:
-
资助金额:$21.58万
-
财政年份:1996
-
负责人:VIRGINIA E. PAPAIOANNOU
-
依托单位:
海外基金