FORESPORE ENGULFMENT DURING B SUBTILIS SPORULATION

描述：本提案的目标是实现详细的 了解枯草杆菌孢子形成的关键步骤， 吞没 在吞噬过程中，孢子形成隔膜的边缘迁移 围绕并完全包围前孢子;最终前孢子完全 封闭在母细胞的细胞质内，并被两个 膜。 吞噬对于孢子形态发生和 晚期孢子形成特异性转录因子的完全激活， 孢子囊的细胞。 虽然几种孢子化特异性 虽然蛋白质与吞噬有关，但其机制仍不清楚。 细菌细胞缺乏与类似蛋白质相关的细胞骨架蛋白。 真核生物事件，因此必须使用一种新的机制来驱动 一个细胞被另一个细胞吞噬。 我们将使用以下具体目标 来加深我们对吞噬的理解 表征已知的吞噬蛋白，以确定其 亚细胞分布，以确定与它们相互作用的蛋白质， 确定其生化活性，并更精确地确定 需要被吞没的阶段。 鉴定吞噬所需的其他蛋白质， 遗传和生物化学方法。 一个系统的发展，以观察吞噬活细菌，使用 视频显微镜和蛋白质融合到绿色荧光蛋白（GFP） 维多利亚水瓶座 希望通过鉴定大量的蛋白质， 吞噬，并通过研究它们的生化活动，相互作用， 亚细胞分布，我们可以开始理解的机制， 一个细菌细胞可以吞噬另一个细菌细胞。 吞噬提供了一个独特的 用于研究细菌中受调节的膜运动的模型系统。

DESCRIPTION: The goal of this proposal is to achieve a detailed understanding of a key step in the sporulation of Bacillus subtilis, engulfment. During engulfment the edges of the sporulation septum migrate around and fully enclose the forespore; ultimately the forespore is fully enclosed within the mother cell cytoplasm and is surrounded by two membranes. Engulfment is essential for both spore morphogenesis and for full activation of late sporulation-specific transcription factors in both cells of the developing sporangium. Although several sporulation-specific proteins have been implicated in engulfment, its mechanism remains obscure. Bacterial cells lack the cytoskeletal proteins associated with similar eukaryotic events, and hence must use a noval mechanism to drive the engulfment of one cell by another. We will use the following specific aims to further our understanding of engulfment: Characterization of the known engulfment proteins to determine their subcellular distribution, to identify proteins with which they interact, to determine their biochemical activities, and to more precisely determine the stage at which they are required for engulfment. Identification of additional proteins required for engulfment, using both genetic and biochemical approaches. The development of a system to observe engulfment in living bacteria, using video microscopy and protein fusions to the green fluorescent protein (GFP) of Aquoria victoria. It is hoped that by identifying a large number of proteins required for engulfment, and by studying their biochemical activities, interactions and subcellular distributions, that we can begin to understand the mechanism by which one bacterial cell can engulf another. Engulfment provides a unique model system for the study of regulated membrane movements in bacteria.