FORESPORE ENGULFMENT DURING B SUBTILIS SPORULATION

描述：这项提案的目标是实现详细的 了解枯草芽孢杆菌产孢子的关键步骤， 被吞没了。在吞噬过程中，孢子形成隔膜的边缘迁移 围绕并完全包围前孔；最终前孔是完全的 包围在母细胞胞质内，并被两个 膜。吞噬作用对孢子形态发生和形成都是必不可少的。 晚期产孢子特异转录因子在两种植物中的完全激活 发育中的孢子囊细胞。尽管有几个特定于孢子形成的 蛋白质与吞噬有关，但其机制仍不清楚。 细菌细胞缺乏与类似蛋白相关的细胞骨架蛋白 真核事件，因此必须使用一种新的机制来驱动 一种细胞吞噬另一种细胞。我们将使用以下具体目标 为了加深我们对吞噬的理解： 已知的吞噬蛋白的特性以确定它们的 亚细胞分布，以确定它们与之相互作用的蛋白质， 测定它们的生化活性，并更准确地测定 他们被要求吞噬的阶段。 使用两种方法鉴定吞噬所需的额外蛋白质 遗传和生化方法。 一种观察活体细菌吞噬情况的系统的开发 视频显微镜与绿色荧光蛋白(GFP)的蛋白质融合 维多利亚水族馆的。 希望通过鉴定大量需要的蛋白质来实现 并通过研究它们的生化活性、相互作用和 亚细胞分布，我们可以通过以下方式开始了解其机制 哪一个细菌细胞可以吞噬另一个细菌细胞。吞没提供了一种独特的 细菌中调节膜运动研究的模型系统。

DESCRIPTION: The goal of this proposal is to achieve a detailed understanding of a key step in the sporulation of Bacillus subtilis, engulfment. During engulfment the edges of the sporulation septum migrate around and fully enclose the forespore; ultimately the forespore is fully enclosed within the mother cell cytoplasm and is surrounded by two membranes. Engulfment is essential for both spore morphogenesis and for full activation of late sporulation-specific transcription factors in both cells of the developing sporangium. Although several sporulation-specific proteins have been implicated in engulfment, its mechanism remains obscure. Bacterial cells lack the cytoskeletal proteins associated with similar eukaryotic events, and hence must use a noval mechanism to drive the engulfment of one cell by another. We will use the following specific aims to further our understanding of engulfment: Characterization of the known engulfment proteins to determine their subcellular distribution, to identify proteins with which they interact, to determine their biochemical activities, and to more precisely determine the stage at which they are required for engulfment. Identification of additional proteins required for engulfment, using both genetic and biochemical approaches. The development of a system to observe engulfment in living bacteria, using video microscopy and protein fusions to the green fluorescent protein (GFP) of Aquoria victoria. It is hoped that by identifying a large number of proteins required for engulfment, and by studying their biochemical activities, interactions and subcellular distributions, that we can begin to understand the mechanism by which one bacterial cell can engulf another. Engulfment provides a unique model system for the study of regulated membrane movements in bacteria.