FORESPORE ENGULFMENT DURING B SUBTILIS SPORULATION

枯草芽孢杆菌孢子形成期间的 Forespore 吞没

• 批准号: 6490150
• 负责人: Kit J Pogliano
• 金额: $ 17.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6490150
• 关键词: Bacillus subtilis; cytoplasm; cytoskeletal proteins; histogenesis; phagocytosis; protein localization; protein structure function; spores; video microscopy

DESCRIPTION: The goal of this proposal is to achieve a detailed understanding of a key step in the sporulation of Bacillus subtilis, engulfment. During engulfment the edges of the sporulation septum migrate around and fully enclose the forespore; ultimately the forespore is fully enclosed within the mother cell cytoplasm and is surrounded by two membranes. Engulfment is essential for both spore morphogenesis and for full activation of late sporulation-specific transcription factors in both cells of the developing sporangium. Although several sporulation-specific proteins have been implicated in engulfment, its mechanism remains obscure. Bacterial cells lack the cytoskeletal proteins associated with similar eukaryotic events, and hence must use a noval mechanism to drive the engulfment of one cell by another. We will use the following specific aims to further our understanding of engulfment: Characterization of the known engulfment proteins to determine their subcellular distribution, to identify proteins with which they interact, to determine their biochemical activities, and to more precisely determine the stage at which they are required for engulfment. Identification of additional proteins required for engulfment, using both genetic and biochemical approaches. The development of a system to observe engulfment in living bacteria, using video microscopy and protein fusions to the green fluorescent protein (GFP) of Aquoria victoria. It is hoped that by identifying a large number of proteins required for engulfment, and by studying their biochemical activities, interactions and subcellular distributions, that we can begin to understand the mechanism by which one bacterial cell can engulf another. Engulfment provides a unique model system for the study of regulated membrane movements in bacteria.

描述：本提案的目标是实现详细的 了解枯草芽孢杆菌孢子形成的关键步骤， 吞没。 在吞没过程中，孢子形成隔膜的边缘迁移 围绕并完全包围前孢子；最终前孢子完全 封闭在母细胞的细胞质内，并被两个 膜。 吞噬对于孢子形态发生和 晚期孢子形成特异性转录因子的完全激活 发育中的孢子囊细胞。 尽管有几种孢子形成特异性 蛋白质与吞噬有关，但其机制仍不清楚。 细菌细胞缺乏与类似的细胞骨架蛋白相关的细胞骨架蛋白。 真核事件，因此必须使用一种新的机制来驱动 一个细胞被另一个细胞吞噬。 我们将采用以下具体目标 为了加深我们对吞没的理解： 已知吞噬蛋白的表征以确定其 亚细胞分布，识别与其相互作用的蛋白质， 确定其生化活性，并更精确地确定 它们需要被吞噬的阶段。 使用两者鉴定吞噬所需的额外蛋白质 遗传和生化方法。 开发一种观察活细菌吞噬的系统，使用 视频显微镜和与绿色荧光蛋白 (GFP) 的蛋白质融合 维多利亚水瓶座。 希望通过鉴定所需的大量蛋白质 吞噬，并通过研究它们的生化活性、相互作用和 亚细胞分布，我们可以通过以下方式开始理解其机制 哪个细菌细胞可以吞噬另一个细菌细胞。 吞没提供了独特的 研究细菌膜运动调节的模型系统。