REGULATION OF CORNEAL WOUND HEALING IN TYPE I DIABETES

I 型糖尿病角膜伤口愈合的调节

• 批准号: 6207738
• 负责人: IAN S ZAGON
• 金额: $ 3.88万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2001-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6207738
• 关键词: clinical research; cornea; corneal epithelium; eye regeneration; growth factor; human subject; immunocytochemistry; in situ hybridization; insulin dependent diabetes mellitus; laboratory rat; opioid receptor; receptor expression; tissue /cell culture; wound healing

DESCRIPTION: (Adapted from the applicant's abstract) The three described specific aims and approach to their accomplishment in this proposal are: 1) to determine the importance of opioid-receptor interaction on homeostatic cellular renewal of the diabetic corneal epithelium by studying OGF/receptor expression and the effects of excess OGF or blockade of OGF activity with naltrexone on DNA synthesis in diabetic rat corneal epithelium limbus and conjunctiva; 2) to define the role of an endogenous opioid system related to growth in the re-epithelialization of denuded corneal epithelium in diabetic rats by examining healing and integrity of the repaired epithelium after systemic and topical treatment with naltrexone of rats subjected to corneal abrasions; and 3) to ascertain the influence of an endogenous opioid system related to growth in the repair of corneal wound healing by studying the effect of opioid receptor blockade on healing of wounded epithelium in human corneas in organ culture.

描述：（改编自申请人的摘要） 本建议的具体目标和实现方法是：1） 确定阿片受体相互作用对稳态细胞的重要性， 通过研究OGF/受体表达的糖尿病角膜上皮更新 以及过量OGF或用纳洛酮阻断OGF活性对 糖尿病大鼠角膜上皮利姆布斯和结膜中的DNA合成; 2） 定义与生长相关的内源性阿片系统的作用， 糖尿病大鼠裸露角膜上皮的再上皮化 检查在全身和全身治疗后修复的上皮的愈合和完整性， 用纳洛酮局部治疗角膜擦伤的大鼠;和 3)确定与生长相关的内源性阿片系统的影响 通过研究阿片类药物在角膜创伤修复中的作用， 受体阻断剂对人角膜上皮损伤愈合的影响 文化

项目成果

Naltrexone, an opioid antagonist, facilitates reepithelialization of the cornea in diabetic rat.

纳曲酮是一种阿片类拮抗剂，可促进糖尿病大鼠的角膜上皮再生。

• DOI: 10.2337/diabetes.51.10.3055
• 发表时间: 2002
• 期刊: Diabetes
• 影响因子: 7.7
• 作者: Zagon,IanS;Jenkins,JoeB;Sassani,JosephW;Wylie,JamesD;Ruth,TorreB;Fry,JamieL;Lang,CMax;McLaughlin,PatriciaJ
• 通讯作者: McLaughlin,PatriciaJ