CHARACTERIZATION OF PEPTIDES/PROTEINS AND OLIGONUCLEOTIDE SYNTHESIS

核心C将表征通过本发明的方法分离的新型肽和蛋白质。 这个计划的项目。 此外，员额的性质和地点 在新的和重组的肽中的翻译修饰， 蛋白质将被识别。 核心还将执行各种 对本计划项目至关重要的服务，包括 合成肽的一级结构测定 重组蛋白的表征和 寡核苷酸 化学序列分析和质谱分析将是 用于表征待研究的肽和蛋白质。 的 从化学序列分析和质谱分析获得的信息 不仅是互补的，而且是协同的，特别是在调查 蛋白质的结构特征。 这种协同作用变得特别重要。 重要的是，当样品的量是有限的，如在 天然产物的特性。 所有这些调查 需要专门的设备和训练有素的人员， 集中在一个核心，以提高效率和促进质量 控制

Core C will characterize novel peptides and proteins isolated by the Projects of this Program. In addition, the nature and site of post translational modifications in both novel and recombinant peptides and proteins will be identified. The Core will also perform a variety of services that are essential for the Projects of this Program, including the primary structure determination of synthetic peptides, the chemical characterization of recombinant proteins and the synthesis of oligonucleotides. Chemical sequence analysis and mass spectrometry will be used to characterize the peptides and proteins to be studied. The information obtained from chemical sequence analysis and mass spectrometry is not only complementary but synergetic, especially when investigating structural features of proteins. This synergy becomes particularly important when the amount of sample is limited such as in the characterization of natural products. As all of these investigations require specialized equipment and well trained personnel they ar best centralized in a Core to increase efficiency and to facilitate quality control.