CHARACTERIZATION OF PEPTIDES/PROTEINS AND OLIGONUCLEOTIDE SYNTHESIS

肽/蛋白质的表征和寡核苷酸合成

• 批准号: 6105169
• 负责人: WOLFGANG FISCHER
• 金额: $ 21.48万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6105169
• 关键词: biomedical facility; mass spectrometry; neuroendocrine system; neuropeptides; nucleic acid chemical synthesis; oligonucleotides; peptide chemical synthesis; posttranslational modifications; protein purification; protein sequence; protein structure

Core C will characterize novel peptides and proteins isolated by the Projects of this Program. In addition, the nature and site of post translational modifications in both novel and recombinant peptides and proteins will be identified. The Core will also perform a variety of services that are essential for the Projects of this Program, including the primary structure determination of synthetic peptides, the chemical characterization of recombinant proteins and the synthesis of oligonucleotides. Chemical sequence analysis and mass spectrometry will be used to characterize the peptides and proteins to be studied. The information obtained from chemical sequence analysis and mass spectrometry is not only complementary but synergetic, especially when investigating structural features of proteins. This synergy becomes particularly important when the amount of sample is limited such as in the characterization of natural products. As all of these investigations require specialized equipment and well trained personnel they ar best centralized in a Core to increase efficiency and to facilitate quality control.

Core C 将表征由 本计划的项目。 此外，邮寄的性质和地点 新型和重组肽的翻译修饰以及 蛋白质将被识别。 该核心还将执行各种 对于本计划的项目至关重要的服务，包括 合成肽一级结构测定，化学 重组蛋白的表征及其合成 寡核苷酸。 化学序列分析和质谱分析将 用于表征待研究的肽和蛋白质。 这 从化学序列分析和质谱中获得的信息 不仅是互补的，而且是协同的，尤其是在调查时 蛋白质的结构特征。 这种协同作用变得特别 当样本量有限时（例如在 天然产物的表征。 由于所有这些调查 需要专门的设备和训练有素的人员，他们是最好的 集中于一个核心以提高效率并促进质量 控制。