CHARACTERIZATION OF PEPTIDES/PROTEINS AND OLIGONUCLEOTIDE SYNTHESIS

核心C将表征通过分离的新肽和蛋白质 本计划的项目。此外，帖子的性质和地点 新型多肽和重组多肽的翻译修饰 蛋白质将被鉴定出来。核心还将执行各种 对本计划的项目至关重要的服务，包括 化学合成肽的一级结构测定 重组蛋白的特性及其合成 寡核苷酸。化学序列分析和质谱分析将 用于表征待研究的多肽和蛋白质。这个 从化学序列分析和质谱学中获得的信息 不仅是互补的，而且是协同的，特别是在调查 蛋白质的结构特征。这种协同作用变得特别 重要的是当样本量有限时，例如在 天然产品的表征。因为所有这些调查 需要专门的设备和训练有素的人员 集中在一个核心中，以提高效率和促进质量 控制力。

Core C will characterize novel peptides and proteins isolated by the Projects of this Program. In addition, the nature and site of post translational modifications in both novel and recombinant peptides and proteins will be identified. The Core will also perform a variety of services that are essential for the Projects of this Program, including the primary structure determination of synthetic peptides, the chemical characterization of recombinant proteins and the synthesis of oligonucleotides. Chemical sequence analysis and mass spectrometry will be used to characterize the peptides and proteins to be studied. The information obtained from chemical sequence analysis and mass spectrometry is not only complementary but synergetic, especially when investigating structural features of proteins. This synergy becomes particularly important when the amount of sample is limited such as in the characterization of natural products. As all of these investigations require specialized equipment and well trained personnel they ar best centralized in a Core to increase efficiency and to facilitate quality control.