LIPID & PROTEIN TRAFFIC & DYNAMICS IN EPITHELIAL CELLS

脂质体

• 批准号: 2856753
• 负责人: MICHAEL A EDIDIN
• 金额: $ 96.5万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2856753
• 关键词: cellular polarity; epithelium; lipid transport; membrane lipids; membrane proteins; protein transport

Epithelial cells, which comprise 50% of all cells in the body [and account for 90% of all human cancers] have as a defining function the creation and maintenance of physiological compartments of the body. Epithelial cells not only physically separate different environments; they regulate selective exchange between them. This requires that the cells' opposing surfaces be functionally differentiated; hence, epithelial cells are functionally polarized. This polarization is accomplished through cooperative formation and dynamic maintenance of junctional complexes between adjacent cells and by the selective or differential delivery and retention of membrane proteins and lipids to one pole or the other each cell. The projects in this program study selective delivery of components to apical and basolateral membrane domains. A variety of perspectives are taken and different techniques are used, but at the same time there is a common focus in all four projects on the Golgi complex, considering its lateral organization and heterogeneity, traffic, composition, and integrity and maintenance of its spatial orientation. Edidin (Project 1) focuses on the lateral organization of Golgi membrane and plasma membrane proteins, particularly GPI-proteins, and lipids, required for the polarized traffic of these molecules. Hubbard (Project 3) focuses on intracellular routes and mechanisms for the polarized delivery and retention of membrane proteins, with emphasis on the endosomal/transcytotic system. Machamer (Project 4) focuses on the mechanisms of the organization and composition of the Golgi complex that important for sorting lipids and proteins destined for the plasma membrane, while maintaining its own integrity. Schroer (Project 6) focuses on the cytoskeletal machinery that maintains the polarized intracellular architecture of epithelial cells and on the routes traveled by vesicles between cytoplasmic membranes and between cytoplasm and surface.

上皮细胞，占体内所有细胞的 50% [和 占所有人类癌症的 90%] 具有定义功能 身体生理区室的创建和维护。 上皮细胞不仅在物理上分隔不同的环境; 它们调节它们之间的选择性交换。 这需要 细胞的相对表面在功能上有所区分；因此， 上皮细胞具有功能性极化。 这种极化是 通过合作形成和动态维护来完成 相邻细胞之间的连接复合物并通过选择性或 膜蛋白和脂质的差异传递和保留 每个电池的一极或另一极。 本计划中的项目 研究选择性地将成分递送至顶端和基底外侧 膜域。 采取各种不同的观点和不同的观点 技术被使用，但同时有一个共同的焦点 高尔基复合体的所有四个项目，考虑到其横向 组织和异构性、流量、组成和完整性 并维持其空间方向。 爱迪丁（项目1） 专注于高尔基膜和血浆的横向组织 需要膜蛋白，特别是 GPI 蛋白和脂质 用于这些分子的极化交通。 哈伯德（项目 3） 重点研究极化的细胞内途径和机制 膜蛋白的传递和保留，重点是 内体/转胞吞系统。 Machamer（项目 4）专注于 高尔基复合体的组织和组成机制 这对于分选运往目的地的脂质和蛋白质非常重要 质膜，同时保持其自身的完整性。 施罗尔 （项目 6）专注于维持细胞骨架的机制 上皮细胞的极化细胞内结构和 囊泡在细胞质膜之间和细胞膜之间移动的路线 细胞质和表面。