MASS SPECTROMETER

质谱仪

• 批准号: 2803467
• 负责人: RICHARD A. GALBRAITH
• 金额: $ 23.65万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803467
• 关键词: bioenergetics; biomedical equipment purchase; clinical biomedical equipment; deuterium; mass spectrometry; protein biosynthesis

Although variety of mass spectroscopic techniques exists, they address specific and limited analytical applications. Measurement of hydrogen stable isotopes (deuterium, 2H, and protium, 1H) has been problematic at low levels of 2H enrichment even by isotope ratio mass spectrometry (IRMS). The limitation of IRMS has been a large sample size requirement and a tedious and complicated purification/reduction scheme necessary to prepare samples as hydrogen gas (H2). This archaic methodology has severely limited the application of 2H-labeled materials as tracers of metabolic processes in the biomedical disciplines. Recent advances using continuous flow IRMS have greatly improved our ability to measure small enrichments of 13C and 15N isotopes in biological materials. These developments have been extended to couple a pyrolysis-reduction oven to an IRMS specially modified for measuring 2H. Availability of such a technique would greatly enhance and expand the research of biomedical investigators at the University of Vermont studying the regulation of metabolism in humans. An additional important feature of the new pyrolysis-IRMS is the rapid measurement of 2H enrichments in physiological water samples for determination of free-living energy expenditure using doubly (2H218O) water administered orally to humans. This technique allows evaluation on the effect of therapies upon energy expenditure in humans in their normal lives and makes it possible to test the efficacy of therapies in large groups of individuals that can be extrapolated to populations of patients. However, large numbers of subjects must be studied using doubly labeled water than our conventional IRMS system can handle. The new pyrolysis-IRMS would allow studies to be performed and measured at reasonable cost in a reasonable period of time in appropriately sized samples of patients. A pyrolysis-IRMS instrument is capable of measuring both low levels of 2H enrichment in both physiological-water samples and in metabolites of in vivo 2H tracer studies is requested for purchase to benefit a variety of investigators.

虽然存在各种质谱技术，但它们针对特定且有限的分析应用。氢稳定同位素（氘，2 H和氚，1H）的测量在低水平的2 H富集下甚至通过同位素比质谱法（IRMS）也存在问题。IRMS的局限性是需要大的样品尺寸和制备氢气（H2）样品所需的繁琐且复杂的纯化/还原方案。这种陈旧的方法严重限制了2 H标记材料在生物医学学科中作为代谢过程示踪剂的应用。使用连续流动IRMS的最新进展极大地提高了我们测量生物材料中13 C和15 N同位素的小富集的能力。这些发展已被扩展到耦合热解还原炉的IRMS专门修改为测量2 H。这种技术的可用性将极大地增强和扩展佛蒙特大学生物医学研究人员研究人类代谢调节的研究。新的热解IRMS的另一个重要特征是快速测量2 H富集的生理水样中的自由生活的能量消耗的测定使用双（2 H218 O）水口服给人类。该技术允许评估治疗对人类正常生活中能量消耗的影响，并使得可以在可以外推到患者群体的大群体中测试治疗的功效。然而，大量的主题必须研究使用双标记水比我们传统的IRMS系统可以处理。新的热解IRMS将允许在合理的时间内以合理的成本在适当大小的患者样本中进行研究和测量。热解IRMS仪器能够测量生理水样品和体内2 H示踪研究的代谢物中的2 H富集的低水平，要求购买以使各种研究人员受益。