NUTRITIONAL EFFECTS ON CARCINOGENESIS--EFFECTS OF FIBER AND FAT ON COLON

营养对致癌的影响--纤维和脂肪对结肠的影响

• 批准号: 6247024
• 负责人: RICHARD A. GALBRAITH
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-23 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247024
• 关键词: carcinogenesis; clinical research; colon neoplasms; dietary fiber; dietary lipid; gastrointestinal epithelium; human subject; nutrition aspect of cancer; nutrition related neoplasm /cancer; nutrition related tag; pathogenic diet

The aim of this protocol remains to try to elucidate the biochemical and cellular mechanisms by which fiber and fat influence the growth, and in some cases the abnormal cancerous growth of the epithelial cells of the colon. Epidemiological evidence clearly indicates that both of these dietary factors play a role in normal colon function and malignant transformation, but the mechanisms of action remain obscure. The approach is to feed normal human volunteers liquid formula diets and to vary the composition of these diets with respect to fat or fiber content and correlate such changes with alterations in specific mRNAs obtained from colon biopsy specimens. The rate of accural of human subjects who are willing to participate remains slow, but since the onset of this protocol in March 1995, 5 patients have sucessfully completed this protocol. All biopsies are being stored in liquid nitrogen pending batch analysis. Future plans are to continue to enroll patients until 8 have been accumulated in each experimental group.

该方案的目的仍然是试图阐明生物化学和 纤维和脂肪影响生长的细胞机制， 在某些情况下，上皮细胞的异常癌性生长， 结肠流行病学证据清楚地表明， 饮食因素在正常结肠功能和恶性结肠功能中起作用。 转化，但作用机制仍然模糊。的 方法是给正常的人类志愿者喂食液体配方食品， 根据脂肪或纤维含量改变这些饮食的组成 并将这些变化与获得的特定mRNA的改变相关联， 从结肠活检标本中分离出来。受试者中， 愿意参与的人仍然很慢，但自从这一事件发生以来， 在1995年3月的方案中，5名患者成功完成了这项研究。 议定书所有活检样本均储存在液氮中等待批次 分析.未来的计划是继续招募患者，直到8例患者 在每个实验组中积累。