NUTRITIONAL EFFECTS ON CARCINOGENESIS--EFFECTS OF FIBER AND FAT ON COLON

营养对致癌的影响--纤维和脂肪对结肠的影响

• 批准号: 6115931
• 负责人: RICHARD A. GALBRAITH
• 金额: $ 3.29万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6115931
• 关键词: carcinogenesis; clinical research; colon neoplasms; dietary fiber; dietary lipid; gastrointestinal epithelium; human subject; nutrition aspect of cancer; nutrition related neoplasm /cancer; nutrition related tag; pathogenic diet

The aim of this protocol remains to try to elucidate the biochemical and cellular mechanisms by which fiber and fat influence the growth, and in some cases the abnormal cancerous growth of the epithelial cells of the colon. Epidemiological evidence clearly indicates that both of these dietary factors play a role in normal colon function and malignant transformation, but the mechanisms of action remain obscure. The approach is to feed normal human volunteers liquid formula diets and to vary the composition of these diets with respect to fat or fiber content and correlate such changes with alterations in specific mRNAs obtained from colon biopsy specimens. The rate of accrual of human subjects who are willing to participate remains slow, but since the onset of this protocol in March 1995, 7 patients have successfully completed this protocol. All biopsies are being stored in liquid nitrogen pending batch analysis. Intermediate analysis of P450 in biopsies was run and analyzed on CDMAS. No significant differences were detected. Future plans are to continue to enroll patients until 8 have been accumulated in each experimental group.

本方案的目的仍然是试图阐明纤维和脂肪影响结肠上皮细胞生长的生化和细胞机制，以及在某些情况下影响结肠上皮细胞的异常癌性生长。 流行病学证据清楚地表明，这两种饮食因素在正常结肠功能和恶性转化中发挥作用，但作用机制仍不清楚。 该方法是喂养正常的人类志愿者液体配方饮食，并改变这些饮食的脂肪或纤维含量的组成，并将这些变化与从结肠活检标本中获得的特定mRNA的变化相关联。 愿意参与的人类受试者的招募速度仍然缓慢，但自1995年3月开始实施本方案以来，已有7例患者成功完成了本方案。 所有活检标本均储存在液氮中，等待批量分析。在CDMAS上运行和分析活组织检查中的P450的中间分析。未检测到显著差异。 未来计划继续招募患者，直至每个实验组累积8例患者。