HIGH PERFORMANCE QUADRUPOLE MS/MS SYSTEM

高性能四极杆 MS/MS 系统

• 批准号: 2791801
• 负责人: DAVID WARSHAWSKY
• 金额: $ 30.9万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2000-12-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791801
• 关键词: analytical chemistry; biomedical equipment; biomedical equipment purchase; environmental health; mass spectrometry

The University of Cincinnati requests funds to purchase a high- performance triple-stage quadruple mass spectrometer/mass spectrometer (MS/MS) system to upgrade its existing capabilities. The University is the twelfth oldest higher educational institutional in the country, and is one of two comprehensive universities in the State of Ohio. With its modern approach to research and education at all levels, it has provided a pool of well-trained scientists, doctors and technical support people now employe across the country. The faculty in the physical and life sciences are at the forefronts of many areas of science and medicine. However, the MS facilities at the University have not kept up with state of the art technology, which is severely hampering the productivity of these researchers. There is currently no instrumentation at the University equivalent to the high-performance triple-stage MS/MS system. This level of MS analysis is critically needed for research and training programs in biological sciences, chemistry, environmental health, molecular genetics, pediatrics, pharmacology, pharmacy, and surgery. The instrument is essential to perform ongoing experiments in NIH-funded projects as well as a future research in fields such as biodegradation and biochemical toxicology, biotransformation and biomarkers of toxin exposure, lipid biochemistry and physiology of pathogenic microbes, and protein peptide structure and chemistry. This instrument will also be used for graduate student and post-doctoral training in various disciplines. A Research I university of the size and funding level of the University of Cincinnati cannot remain competitive in attaining its long-term educational and advanced research goals without such instrumentation. The requested instrumentation will enhance sensitivity and detection levels, and reduce noise levels, features which current instruments at the University cannot attain. The instrument will be able to provide mass spectral data for large biomolecules. The ionization dn sample introduction methods will enable the analysis of heat-labile large and intact biomolecules. Hydrolysis and derivitization of large molecules required by currently available instrumentation can alter the structure of these molecules, hence scientific journals are increasingly demanding intact molecule analyses. The requested instrument can detect either positive or negative ions formed by highly efficient ion ionization methods. This will improve the ability to conduct studies such as the structural identification of signature lipids and enable subsequent sensitive detection of pathogens and toxin degraders present in humans and the outdoor environment. The instrument will be able to analyze complexes such as polycyclic aromatic hydrocarbon-DNA adducts, and aid in the elucidation of active sites of enzymes, pumps, drugs and receptors. These data may lead to the development of new drugs and their derivatives and the identification of biodegradation products of environmental pollutants. There are several ongoing projects across the University that are only possible to complete or advance with updated MS capabilities; beyond the magnetic sector double-focusing gas chromatography/mass spectrometers currently available.

辛辛那提大学申请资金购买高性能三级四重质谱仪/质谱仪（MS/MS）系统，以升级其现有能力。该大学是美国第十二古老的高等教育机构，也是俄亥俄州两所综合性大学之一。凭借其在各级研究和教育的现代方法，它提供了一批训练有素的科学家，医生和技术支持人员，现在在全国各地就业。在物理和生命科学的教师是在科学和医学的许多领域的前沿。然而，该大学的MS设施没有跟上最先进的技术，这严重阻碍了这些研究人员的生产力。目前在大学没有相当于高性能三级MS/MS系统的仪器。这种水平的MS分析是迫切需要的研究和培训计划，在生物科学，化学，环境卫生，分子遗传学，儿科，药理学，药学和外科。该仪器对于在NIH资助的项目中进行正在进行的实验以及未来在生物降解和生物化学毒理学，毒素暴露的生物转化和生物标志物，病原微生物的脂质生物化学和生理学以及蛋白质肽结构和化学等领域的研究至关重要。该仪器还将用于各学科的研究生和博士后培训。一个研究型大学的规模和资金水平的辛辛那提大学不能保持竞争力，在实现其长期的教育和先进的研究目标，没有这样的仪器。所要求的仪器将提高灵敏度和检测水平，并降低噪音水平，这是该大学目前仪器无法实现的功能。该仪器将能够提供大生物分子的质谱数据。离子化和样品引入方法将使热不稳定的大而完整的生物分子的分析成为可能。目前可用的仪器所需的大分子的水解和衍生化可以改变这些分子的结构，因此科学期刊越来越要求完整的分子分析。 所要求的仪器可以检测由高效离子电离方法形成的正离子或负离子。这将提高进行特征脂质结构鉴定等研究的能力，并能够随后灵敏地检测人类和户外环境中存在的病原体和毒素降解剂。该仪器将能够分析多环芳烃-DNA加合物等复合物，并有助于阐明酶、泵、药物和受体的活性位点。这些数据可能会导致新药物及其衍生物的开发和环境污染物的生物降解产物的鉴定。整个大学有几个正在进行的项目，只有更新MS功能才能完成或推进;超越目前可用的磁扇区双聚焦气相色谱/质谱仪。